Arbo

CRL-3631 ^™

Arbo was established from a NOTCH2 R2400*-mutated blastoid MCL cell line, isolated from a malignant pleural effusion of a patient with MCL (Lymphoma; Mantle Cell; Stage IV, high grade). These cells may be used in the investigation of pediatric medulloblastoma and neuroscience research.

Product category: Human cells
Organism: Homo sapiens, human
Morphology: Lymphoblast-like
Tissue: Peripheral blood; Pleural effusion
Disease: Lymphoma; Mantle Cell
Applications: Cancer research

Neuroscience
Product format: Frozen
Storage conditions: Vapor phase of liquid nitrogen

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Dulbecco's Modified Eagle's Medium (DMEM)

30-2002

Fetal Bovine Serum (FBS)

30-2020

Dimethylsulfoxide (DMSO)

4-X

General

Specific applications: Pediatric cancer research, cancer research, neuroscience

Characteristics

Cells per vial: Approximately 5.0 to 7.0 x 10⁶
Growth properties: Suspension, cells grow in loose clumps
Age: 69 years
Ethnicity: White
Gender: Male
Karyotype: (51, XY, +1, add(2)(p11.2), +7, add(8)(p11.2), −10, add(11)(p11.2), t(11;14)(q13;q32),+add(13)(q34), del(13)(q14q14)x1- 2, add(18)(p11.2),−22,+r, +1, −3mar[cp22])
Tumorigenic: Yes; This cell line will grow as xenografts in immunosuppressed mice.
Oncogene: Depositor states that cells positive for CD5, CD19, CD20, CD22, and CD23, and negative for CD2, CD3, CD7, CD10, and CD71. Cells contain NOTCH2 R2400* mutation with expression of CD23. Cells are resistant to venetoclax.
Comments: Arbo cells were isolated by obtaining a sample of pleural effusion from a 69 year old Caucasian male. Mononuclear cells were then isolated from the sample by Ficoll density gradient centrifugation.

Handling information

Complete medium

The base medium for this cell line is ATCC-formulated DMEM (ATCC 30-2002). To make the complete growth medium, add the following components to the base medium:

Fetal bovine serum (FBS; ATCC 30-2020) to a final concentration of 10%
L-Glutamine (ATCC 30-2214) to a final concentration of 2 mM

Temperature

37°C

Atmosphere

95% Air, 5% CO₂

Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.

Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 to 400 x g for 8 to 12 minutes.
Carefully aspirate the supernatant and discard, leaving the cell pellet.
Gently resuspend the cell pellet in 15 mL fresh pre-warmed complete growth medium, and transfer cell suspension into a vented, T75 flask.
Place the flask in a 37°C incubator with 5% CO₂ .

Subculturing procedure

Re-seed cells at log phase every 2-5 days when there are numerous, healthy-appearing clusters present in suspension. Pre-warm fresh growth medium prior to use. Swirl the flask gently to evenly distribute cells in medium.
Cultures can be maintained by addition or replacement of fresh medium. Do not over dilute cell culture, it is recommended to add equal volumes of fresh media and perform full changes via centrifugation if media appears acidic.
1. For acidic cultures, centrifuge cells for 5-12 min at 170 to 400 x g.
2. Discard spent media and re-suspend cell pellet in pre-warmed fresh complete growth media.
3. Pipette cells gently to break aggregates.
4. Add the cell suspension to a vented flask.
5. Place the flask in a 37°C incubator with 5% CO2
Note: Due to large, tight clusters formed by these cells, it may be difficult to accurately measure cell number and viability. Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.

Reagents for cryopreservation

Complete culture medium + 5% DMSO (ATCC 4-X)

Quality control specifications

Bacterial and fungal testing: Not detected
Mycoplasma contamination: Not detected
Virus testing: Cytomegalovirus (CMV): Not detected

Epstein-Barr virus (EBV): Not detected

Hepatitis B virus (HBV): Not detected

Human Immunodeficiency virus (HIV): Not detected

Human papillomavirus (HPV): Not detected

History

Depositors: Dr. Nakhle Saba, MD
Tulane University
Deming Department of Medicine, Section of Hematology & Medical Oncology
1430 Tulane Ave. #8578, New Orleans, LA 70112
Year of origin: 2017

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

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