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Cells contain Human papillomavirus type 18 (HPV-18) sequences
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
WPE1-NB14 cells belong to a family of cell lines, referred to as the MNU cell lines, which are all derived from RWPE-1 cells after exposure to MNU. The larger family of cell lines, including RWPE-1 cells with a common lineage, mimics multiple steps in progression from normal epithelium to prostatic intra-epithelial neoplasia, and then to invasive cancer. The MNU cell lines, in order of increasing malignancy are: WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850), WPE1-NB11 (ATCC CRL-2851), and WPE1-NB26 (ATCC CRL-2852).
WPE1-NB14 cells show moderate invasive ability in the in vitro Boyden chamber invasion assay RefWebber MM, et al. Human cell lines as an in vitro/in vivo model for prostate carcinogenesis and progression. Prostate 47: 1-13, 2001. PubMed: 11304724.
The colony forming efficiency (CFE) of 1.85% and the invasive ability of WPE1-NB14 cells are both greater than those of WPE1-NA22 cells (ATCC CRL-2849) RefWebber MM, et al. Human cell lines as an in vitro/in vivo model for prostate carcinogenesis and progression. Prostate 47: 1-13, 2001. PubMed: 11304724.
The cells form small tumors after subcutaneous injection. The tumors are a little larger than those formed by WPE1-NA22 cells (ATCC CRL-2849).
The depositor report that the parent RWPE-1 cell line (ATCC CRL-11609) was screened for Hepatitis B and C, and human immunodeficiency viruses, and was found to be negative.
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Note:Subculture cells before they reach confluence. Do not allow cells to become confluent.
Note: Cells grown under serum-free or reduced serum conditions may not attach strongly during the 24 hours after subculture and should be disturbed as little as possible during that period.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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Bello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605
Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606
Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420
Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548
Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636