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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
The cells are interleukin-2 (Il-2) dependent. They are positive for IL-2 receptor, feline CD3, feline CD4 and major histocompatibility complex (MHC) class II antigen. They are negative for feline CD8.
FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells. RefMiyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760
Note: It is not necessary to remove the cryoprotective agent. If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information.
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Miyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760
Miyazawa T, et al. Further characterization of a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive for feline immunodeficiency virus. J. Vet. Med. Sci. 54: 173-175, 1992. PubMed: 1313703