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Cells contain Hepatitis B virus (HBV)
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
SNU-398 was derived in 1990 by J.-G. Park and associates from an anaplastic hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C.
Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum.
Grossly, the original tumor was single nodular with perinodular extensions.
Histologically, it was trabecular type.
The cultured cells are multinucleated, and maintain the production of intracytoplasmic hyaline globules as seen in the original tumor.
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This material was deposited by Korean Cell Line Bank and is distributed for research purposes only under the ATCC Material Transfer Agreement. This material has been released subject to the following:
Any proposed commercial use of these cells must first be negotiated with:
Korean Cell Line Bank
Chongno-gu, Seoul, 110-744 Korea
Phone: (02) 760-3380