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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information.
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Yoneda T, Pratt RM. Mesenchymal cells from the human embryonic palate are highly responsive to epidermal growth factor. Science 213: 563-565, 1981. PubMed: 7017936
Yoneda T, Pratt RM. Interaction between glucocorticoids and epidermal growth factor in vitro in the growth of palatal mesenchymal cells from the human embryo. Differentiation 19: 194-198, 1981. PubMed: 6458523
Yoneda T, Pratt RM. Glucocorticoid receptors in palatal mesenchymal cells from the human embryo: relevance to human cleft palate formation. J. Craniofacial Genet. Dev. Biol. 1: 411-423, 1981.
Pratt RM, et al. Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate. Teratog. Carcinog. Mutagen. 2: 313-318, 1982. PubMed: 6130630