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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Adaptation to other types of sera generally results in slow growth with a prolonged lag in growth.
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
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American Association of Anatomists. Sixty-ninth annual session; Marquette University School of Medicine, Milwaukee, Wisconsin. Anat. Rec. 124: 490, 1956.
Hull RN, et al. Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research. J. Exp. Med. 115: 903-918, 1962. PubMed: 14449901
Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902