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Borrelia miyamotoi Fukunaga et al.

BAA-3151

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Borrelia miyamotoi strain LB-2001 is a whole-genome sequenced bacterium that was isolated from a tick in the United States. It has applications in vector-borne disease and infectious disease research.
Product category
Bacteria
Classification
Bacteria, Spirochaetes, Spirochaetia, Spirochaetales, Borreliaceae
Strain designation
LB-2001
Type strain
No
Genome sequenced strain
Yes
Isolation source
Tick (Ixodes scapularis)
Geographical isolation
United States
Applications
Vector-borne disease research
Infectious disease research
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Comments
Depositor states that the strain is thought to have variable major proteins (Vmps) responsible for gene switching to avoid host antibody responses and cause relapsing fever similar to other members of relapsing fever Borrelia.

Handling information

Medium
Temperature
35°C
Atmosphere
Microaerophilic
Handling procedure
  1. Open thawed vial.
  2. Aseptically transfer the entire contents to a 5-6 mL tube of #3012 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
  3. Use several drops of the primary broth tube to inoculate a #260 plate.
  4. Incubate at 35°C under microaerophilic conditions for 7-10 days. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack or other acceptable method. All tubes and slants should be incubated with caps loosened.
Handling notes
It takes between 7-10 days for the culture to show active growth. Acid formation will change the medium to a light or yellow orange color.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Borrelia miyamotoi
Depositors
Gilmore R
Type of isolate
Arthropod
Cross references
GenBank GCF_017748085.1 Borrelia miyamotoi (spirochetes) complete genome
GenBank NC_022079.2 Borrelia miyamotoi LB-2001, complete sequence
GenBank CP010328.2 Borrelia miyamotoi LB-2001 plasmid lpB sequence
GenBank KR869094.1 Borrelia miyamotoi strain LB-2001 plasmid lpD, partial sequence
GenBank KU041636.1 Borrelia miyamotoi strain LB-2001 plasmid lpE, partial sequence
GenBank KU145468.1 Borrelia miyamotoi strain LB-2001 plasmid lpA PF50-like protein, PF32-like protein, and PF-49-like protein genes, complete cds
GenBank KT355574.1 Borrelia miyamotoi LB-2001 plasmid cpD, complete sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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Frequently Asked Questions

References

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