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Vibrio campbellii (Baumann et al.) Baumann et al.

BAA-1116

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This is one of several strains (ATCC® BAA-1116™ through ATCC® BAA-1121™) included in a "quorum sensing reporter kit" developed by the depositor. Wild type strain that responds to both AI-1 and AI-2 autoinducers.
Product category
Bacteria
Strain designation
BB120
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Marine
Applications
Bioinformatics
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
quorum sensing bacterium
Preceptrol
No

Characteristics

Comments
This is one of several strains (ATCC BAA-1116 through ATCC BAA-1121) included in a "quorum sensing reporter kit" developed by the depositor. When using this strain as part of the quorum sensing kit or in any other bioluminescent assays, Autoinducer Bioassay medium should be used (see product information sheet).
Genome sequenced strain

Vibrio harveyi BAA-1116 (and its derived mutants in panel MP-6) has been reclassified as Vibrio campbellii based on microarray comparative genome hybridization and multilocus sequence analysis. RefLin B, et al. Comparative genomic analyses identify the Vibrio harveyi genome sequenced strains BAA-1116 and HY01 as Vibrio campbellii. Environ. Microbiol. Rep. 2(1): 81-89, 2010. PubMed: 20686623

Handling information

Medium
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #2 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #2 agar slant and/or plate.
  5. Incubate the tubes and plate at 30°C for 24 hours.
Handling notes

When using this strain as part of the "quorum sensing kit" or in any other bioluminescent assays, #2746 media should be used. Marine media contains boric acid and may cause otherwise bioluminescent-negative strains to luminesce, as boron is a key element in autoinducer-2.

This strain exhibits bioluminescence in #2746 media.
This is the wild-type strain for the quorum sensing reporter kit.
Purified genomic DNA of this strain is available as ATCC® BAA-1116D-5™.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Vibrio harveyi (Johnson and Shunk) Baumann et al.
Depositors
BL Bassler
Type of isolate
Environmental
Year of origin
1993
Special collection
NSF - Bacteriology
Cross references
GenBank CP000790 Vibrio harveyi ATCC BAA-1116 chromosome II, complete sequence
GenBank CP000791 Vibrio harveyi ATCC BAA-1116 plasmid pVIBHAR, complete sequence
GenBank CP000789 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Bassler BL, et al. Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. J. Bacteriol. 179: 4043-4045, 1997. PubMed: 9190823

Lin B, et al. Comparative genomic analyses identify the Vibrio harveyi genome sequenced strains BAA-1116 and HY01 as Vibrio campbellii. Environ. Microbiol. Rep. 2(1): 81-89, 2010. PubMed: 20686623

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