Human Coxsackievirus B4 (ATCC® VR-184)

Classification: Picornaviridae, Enterovirus, Enterovirus B  /  Product Format: frozen 1.0 mL

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Classification Picornaviridae, Enterovirus, Enterovirus B
Deposited As Coxsackievirus B 4
Agent Human Coxsackievirus B4
Strain J.V.B. (Benschoten)
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Stool from 10-year-old child, New York, 1951
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-184 images
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
LLC-MK2 Derivative (ATCC® CCL-7.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor H Plager
Source Stool from 10-year-old child, New York, 1951
Year of Origin 1951

Dalldorf G. The Coxsackie viruses. Bull NY Acad.. Med 26: 329-335, 1950. PubMed: 15411582

Sickles GM, et al. Isolation and type determination of Coxackie virus, group B, in tissue culture. Proc. Soc. Exp. Biol. Med. 88: 22-24, 1955. PubMed: 14357332

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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