Human respiratory syncytial virus, Purified (ATCC® VR-1540P)

Classification: Paramyxoviridae, Pneumovirus  /  Product Format: frozen 200 µL per vial

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Classification Paramyxoviridae, Pneumovirus
Deposited As Respiratory syncytial virus
Agent Human respiratory syncytial virus, Purified
Strain A2
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 200 µL per vial
Storage Conditions  -70°C or colder
ATCC® VR-1540P™ is a purified virus product suspended in 200 µL of Hank's Balanced Salt Solution with Ca2+, Mg2+ and 25 mM HEPES Buffer. ATCC® VR-1540™ is prepared from original isolate deposited as ATCC® VR-1302™, which was grown in presence of neutralizing serum to remove contaminating adenovirus type 1.
Effect on Host
CPE, syncytial formation, rounding, and sloughing
Recommended Host
HeLa (ATCC® CCL-2)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an MOI 0.1. Adsorb for 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 3 to 10 days

Effect on Host
CPE, syncytial formation, rounding, and sloughing
Name of Depositor ATCC, L Potash
Source Lower respiratory tract of infant with bronchiolitis and bronchopneumonia, Melbourne, Australia, 1961

Cameron R, et al. Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302). Virus Res. 92: 151-156, 2003. PubMed: 12686423

Lewis FA, et al. A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Med. J. Aust. 2: 932-933, 1961.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation