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Dengue virus type 4 (ATCC® VR-1490)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen

Permits and Restrictions

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Classification Flaviviridae, Flavivirus
Agent Dengue virus type 4
Strain H241 (TC adapted)
Applications
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder
Comments
This virus appears to be one cause of Southeast Asian mosquito-borne hemorrhagic fever and has also been associated with classical Dengue fever. Many additional strains have been isolated from serum of human patients and Aedes aegypti and Aedes albopictus.
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture (focal cell degeneration and inhibited cell growth)
CPE in TC (focal cell degeneration and inhibited cell growth); death of sM
Yes, in vivo effects: death of suckling mice
Recommended Host

Host of Choice: RhMkK TC

Host Range:  sM (i.c. or i.p.); RhMk (i.c.); MkK TC

Growth Conditions
Duration: 4-10 days at 37C in tissue culture; 4-6 days in suckling mice
Cryopreservation
Storage temperature: -70C or lower
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture (focal cell degeneration and inhibited cell growth)
CPE in TC (focal cell degeneration and inhibited cell growth); death of sM
Yes, in vivo effects: death of suckling mice
Name of Depositor W Brandt, ATCC
Special Collection NCRR Contract
Source
derived from existing strain
References

Hammon WM, et al. Viruses associated with epidemic hemorrhagic fevers of the Philippines and Thailand. Science 131(3407): 1102-1103, 1960. PubMed: 14399343

Hammon WM, et al. New hemorrhagic fevers of children in the Philippines and Thailand. Trans Assoc Am Physicians 73:140-155, 1960. PubMed: 13711073

Derived at the ATCC from ATCC VR-217 (mouse passaged material, no longer available) by tissue culture passage in LLC-MK2 cells.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation