Comments
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The Adult Melanocyte Growth Kit contains components that when added to Dermal Cell Basal Medium (ATCC PCS-200-030) create a complete low-serum culture environment for adult melanocytes derived from normal human epidermis (e.g., Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013). The complete low serum medium is formulated to support robust adult melanocyte growth in the absence of cholera toxin and phorbol 12-myristate 13-acetate (PMA). No feeder layers, extracellular matrix proteins or other substrares are required.
The final concentration for each component in complete adult melainocyte growth medium is as follows:
- rh Insulin: 5 µg/mL
- Ascorbic Acid: 50 µg/mL
- L-Glutamine: 6 mM
- Epinephrine: 1.0 µM
- Calcium chloride: 1.5 mM
- Peptide Growth Factor: 0.2%, Proprietary formulation
- M8 Supplement: 1%, Proprietary formulation
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