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Organism: Homo sapiens, human  /  Cell Type: endothelial  /  Tissue: aorta  /  Disease: normal

Permits and Restrictions

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Organism Homo sapiens, human
Tissue aorta
Cell Type endothelial
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 2  [Cells containing SV40 viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 34 years old
Gender female

TeloHAEC-GFP (ATCC CRL-4054) cells retain important endothelial cell characteristics such as  CD31/PECAM-1 marker expression and LDL functional uptake; the cells also show effective inflammatory response upon TNFα treatment and increase proliferation upon VEGF stimulation.  When co-cultured with fibroblasts, TeloHAEC cells can also form neoangiogenic tubular networks in vitro, which are responsive to VEGF stimulation and suramin inhibition.

Storage Conditions liquid nitrogen vapor phase
Karyotype This is a diploid cell line of female origin with a consistent normal kayrotype at low and high passages
Images CRL-4054 Cell Micrograph CRL-4054 TeloHaec-GFP CD31 Positive CRL-4054 TeloHaec-GFP AcLDL Uptake CRL-4054 TeloHaec-GFP co-cultured with hTERT MSC - Tubulogenesis
Derivation TeloHAEC-GFP is a clonal cell line by stably expressing EmGFP under EF1α promoter in TeloHAEC (ATCC CRL-4052) cell line.
Clinical Data female 


Antigen Expression Positive for CD31/PECAM-1 expression  and capable of uptaking Low Density Lipoprotein (LDL).
Complete Growth Medium The base medium for this cell line is Vascular Cell Basal Medium (ATCC PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041).   Optional: Add 0.3ug/mL Puromycin (Santa Cruz Biotech: sc-108071A). Note: Do not filter complete medium.
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes.

Subculture when the culture is about 90% confluent.
  1. Remove and discard spent medium.
  2. Briefly rinse the cells with Dulbecco's Phosphate Buffered Saline (DPBS, ATCC 30-2200) and discard rinse solution.
  3. Add 2.0 to 3.0 mL room temperature Trypsin-EDTA for Primary Cells (ATCC PCS-999-003) to the flask. Incubate at 37°C for 5 min (until cells have detached).
  4. Neutralize trypsin by adding an equal volume of room temperature 2% FBS in DPBS.
  5. Centrifuge cells at 250 x g for 5 min at room temperature.
  6. Remove supernatant. Resuspend pellet in 6.0 to 8.0 mL Complete Growth Medium.
  7. Count cells, and seed 5.0 x 103 to 8.0 x 103 viable cells/cm2 to new culture vessels.
Medium Renewal: Every 2-3 days.
Cryopreservation 90% FBS, 10% DMSO
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile

D5S818:  12        
D13S317: 9, 12
D7S820: 10, 11        
D16S539: 12, 13
vWA: 15, 16             
Amelogenin:  X
CSF1PO: 11, 12
TH01: 6, 8
Population Doubling Level (PDL) As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
Name of Depositor ATCC
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
  1. Antibiotics in culture medium for TeloHAEC-GFP cells

    Date Updated: 4/8/2015


For commercial accounts, this cell line is only distributed under the terms of a fully signed and executed ATCC® Material Transfer Agreement and Addendum. If the commercial account is screening per completed Addendum, the recipient will be required to pay a Screening Fee (ATCC® ACS-2103F™).

Screening Use is defined as use of Biological Material in small molecule and biologic drug discovery, including initial target identification and validation, assay development, high throughput screening, hit identification, lead optimization, and selection of candidates for clinical development.

If the commercial account is not screening per the completed Addendum, the recipient will not be required to pay a Screening Fee.