U-CH12 (ATCC® CRL-3326)

Organism: Homo sapiens, human  /  Tissue: primary: sacrum  /  Disease: chordoma

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Organism Homo sapiens, human
Tissue primary: sacrum
Product Format frozen 1.0 mL
Morphology mesenchymal
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease chordoma
Age 83
Gender male
Ethnicity White
Applications
Use as a model for chordoma for drug development

The Chordoma Foundation may be able to offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information.
Storage Conditions liquid nitrogen vapor phase
Images Cell Micrograph of U-CH12, ATCC CRL-3326
Comments Nuclear expression of brachyury and CD24 (verified with immunofluorescence and PCR)
Complete Growth Medium

The base medium for this cell line is Iscove's Modified Dulbecco's Medium (IMDM; ATCC® No. 30-2005): RPMI-1640 Medium (ATCC® No. 30-2001) at a 4 to 1 ratio. To 500 mL IMDM:RPMI 1640 (4:1) add the following components to make the complete medium:

Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with DPBS (ATCC catalog # 30-2200) to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    Cultures can be established between 2x 104 and 6 x 104 viable cells/cm2.
  6. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 2 X 104 and 1.3 X 105 cell/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial Approximately 2 to 2.5 x 106 cells
Volume 1.0 mL
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 15
D5S818: 11,12
D7S820: 8,12
THO1: 9.3
TPOX: 8,11
vWA: 16,17
Note: Amelogenin Y was present in original depositor material but was lost after cells were in culture for several passages.
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viability ≥ 50%
Population Doubling Time 11-13 days
Name of Depositor P Moller, University of Ulm, Germany
Year of Origin 2014
References

von Witzleben A, et al. Preclinical characterization of novel chordoma cell systems and their targeting by pharmocological inhibitors of the CDK4/6 cell-cycle pathway. Cancer Res 75(18): 3823-31. PubMed: 26183925

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

von Witzleben A, et al. Preclinical characterization of novel chordoma cell systems and their targeting by pharmocological inhibitors of the CDK4/6 cell-cycle pathway. Cancer Res 75(18): 3823-31. PubMed: 26183925