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Aedes albopictus clone C6/36 (ATCC® CRL-1660™)

Organism: Aedes albopictus, mosquito, Asian tiger  /  Tissue: larva, whole  / 

Permits and Restrictions

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Organism Aedes albopictus, mosquito, Asian tiger
Tissue
larva, whole
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age larva
Applications
This cell line is useful for the replication of flaviviruses and reportedly can be used to replicate Dengue viruses to high titers. They are also a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype diploid
Images ATCC CRL-1660 Cell Micrograph
Tumorigenic No
Effects
Yes, did form colonies in semisolid medium
No, the cells were not tumorigenic in immunosuppressed mice
Comments
The cells are non-anchorage dependent, are not tumorigenic and maintain a diploid chromosome number.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultures are prepared by scraping or by vigorous pipetting. Remove the old medium, add fresh complete culture medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 28°C
Name of Depositor KH Eckels
Deposited As Aedes albopictus
References

Igarashi A. Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J. Gen. Virol. 40: 531-544, 1978. PubMed: 690610

Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967.

Clone C6/36 was derived from A. albopictus cells (see ATCC CCL-126) which were adapted to Eagle's minimum essential medium by A. Igarashi and then cloned and recloned by seeding single cell suspensions into petri dishes.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
Other Documentation
Other Document
Cell Micrograph
FAQ's
  1. ATCC CRL-1660 culture conditions


    Date Updated: 12/18/2017

References

Igarashi A. Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J. Gen. Virol. 40: 531-544, 1978. PubMed: 690610

Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967.

Clone C6/36 was derived from A. albopictus cells (see ATCC CCL-126) which were adapted to Eagle's minimum essential medium by A. Igarashi and then cloned and recloned by seeding single cell suspensions into petri dishes.