Complete Growth Medium
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The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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Subculturing
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Subcultures are prepared by scraping or by vigorous pipetting. Remove the old medium, add fresh complete culture medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
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Cryopreservation
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Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
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Culture Conditions
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Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 28°C
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