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CCD-34Lu (ATCC® CRL-1491™)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: lung  /  Disease: normal

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Age 2.5 weeks
Gender female
Ethnicity Black
Applications
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497.
Derivation
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497.
Clinical Data
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497.
female
Black
2.5 weeks
Comments
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility).
A normal skin line derived from the same patient is available as ATCC CRL-1497.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,13
D13S317: 9,13
D16S539: 8,13
D5S818: 12
D7S820: 10
TH01: 7,9
TPOX: 11
vWA: 15,16
Name of Depositor GP Maxwell
Deposited As Homo sapiens
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS