Detroit 532 (ATCC® CCL-54)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number 47; trisomic group 21; Down Syndrome
Clinical Data
Cells senesce after approximately 30 serial subcultivations counted from the tissue of origin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium:
  • lactalbumin hydrosolate 0.1% (final conc.)
  • fetal bovine serum 10% (final conc.)
  • Subculturing
    Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
    Medium Renewal: Every 2 to 3 days
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37.0°C
    STR Profile
    Amelogenin: X,Y
    CSF1PO: 12
    D13S317: 11,13
    D16S539: 10,13
    D5S818: 9,12
    D7S820: 8
    THO1: 7,9
    TPOX: 8
    vWA: 16,18
    G6PD, B
    Name of Depositor CS Stulberg
    Year of Origin 1964
    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation