clone 1-5c-4 [Wong-Kilbourne derivative (D) of Chang conjunctiva] (ATCC® CCL-20.2)

Organism: Homo sapiens, human  / 

Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain human papilloma virus (HPV-18)

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This line was originally thought to be derived from normal conjunctiva, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was originally thought to be derived from normal conjunctiva, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
HeLa Markers Y
Genes Expressed
keratin,The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
keratin
Comments
This line was originally thought to be derived from normal conjunctiva, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
This cell line contains HeLa marker chromosomes and has type A G6PD (see Nature 259:211-213, 1976 and In Vitro 14:469-475, 1978).
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Complete Growth Medium The base medium for this cell line is Medium 199 containing Earle's BSS and 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following component to the base medium: bovine calf serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor ED Kilbourne
References

Wong SC, Kilbourne ED. Changing viral susceptibility of a human cell line in continuous cultivation. I. Production of infective virus in a variant of the Chang conjunctival cell following infection with swine or N-WS influenza viruses. J. Exp. Med. 113: 95-110, 1961. PubMed: 13786478

Chang RS. Continuous subcultivation of epithelial-like cells from normal human tissues. Proc. Soc. Exp. Biol. Med. 87: 440-443, 1954. PubMed: 13237268

. . Virology 26: 478, 1965.

Gomez-Duarte OG, et al. Binding of vitronectin to opa-expressing Neisseria gonorrhoeae mediates invastion of HeLa cells. Infect. Immun. 65: 3857-3866, 1997. PubMed: 9284164

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

Basic Documentation
References

Wong SC, Kilbourne ED. Changing viral susceptibility of a human cell line in continuous cultivation. I. Production of infective virus in a variant of the Chang conjunctival cell following infection with swine or N-WS influenza viruses. J. Exp. Med. 113: 95-110, 1961. PubMed: 13786478

Chang RS. Continuous subcultivation of epithelial-like cells from normal human tissues. Proc. Soc. Exp. Biol. Med. 87: 440-443, 1954. PubMed: 13237268

. . Virology 26: 478, 1965.

Gomez-Duarte OG, et al. Binding of vitronectin to opa-expressing Neisseria gonorrhoeae mediates invastion of HeLa cells. Infect. Immun. 65: 3857-3866, 1997. PubMed: 9284164

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830