ROCK Inhibitor Y27632
ATCC determined that a biosafety level is not applicable to this material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to complete your own risk assessment and understand any potential hazards associated with the material per your organization’s policies and procedures and any other applicable regulations as enforced by your local or national agencies.
Preparation and storage
Prepare a 10 mM stock solution
B. Conditional reprogramming of cells (CRC) culture
Refer to the ATCC Stem Cell Culture Guide for more information.
Cell culture medium: Pluripotent Stem Cell SFM XF/FF (ATCC ACS-3002) is recommended for feeder free culture and ROCK Inhibitor Y-27632 is required for initial culture of iPSCs.
Preparation of Medium Supplemented with ROCK Inhibitor Y-27632
Handling Procedure for Frozen Cells and Initiation of Cultures
Note: The use of ROCK inhibitor may cause a transient spindle-like morphology effect on iPSCs. However, the colony morphology will recover after subsequent media change without ROCK inhibitor
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