SW 1783 [SW-1783, SW1783]

1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Initiate culture as soon as possible upon receipt.

1. Thaw by rapid agitation in 37°C water bath. Thawing should be rapid (within 40-60 seconds). As soon as the ice is melted, remove the ampule from the waterbath. All of the operations from this point on should be carried out under strict aseptic conditions.
2. Transfer the cell suspension and dilute it with the recommended culture medium in a culture flask (see specific batch information above for dilution ratio); incubate at 37°C in a closed system without CO₂ in air. Since it is important to avoid excessive alkalinity of the medium during recovery of the cells, it is suggested that the culture medium be placed into the culture flask, tube, etc. and the pH be adjusted, as necessary, prior to the addition of the vial contents. Note that the bicarbonate content of the culture medium will determine whether an atmosphere containing CO2 will be required
3. It is not necessary to remove the freezing additive. However, if desired, the culture medium may be changed to remove the protective freezing additive (dimethylsulfoxide) 24 hours after thawing. If it is desired that the freezing additive be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the above diluted suspension at approximately 125 x g for 10 minutes, discard the fluid and resuspend the cells with growth medium at the dilution ratio given in the specific batch information above.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

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