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Quantitative Genomic DNA from Clostridioides difficile strain 90556-M6S

9689DQ

Quantitative Genomic DNA from Clostridioides difficile strain 90556-M6S can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Product category
Bacteria
Product type
Molecular standard
Nucleic acid
Derived from
90556-M6S [L.S. McClung 1780] (ATCC 9689)
Organism
Clostridioides difficile (Prevot) Lawson et al.
Type strain
Yes
Genome sequenced strain
Yes
Applications
Assay development
Enteric disease research
Infectious disease research
Specification range
≥1 x 105 copies/μL
Volume
100 μL
Product format
Frozen
Storage conditions
-20°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
ATCC Genuine Nucleics can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.

Characteristics

Toxin genes
cdtB (Binary toxin) negative
tcdA (Toxin A) positive
tcdB (Toxin B) positive
Comments
Genomic DNA isolated from a preparation of Clostridioides difficile strain 90556-M6S (ATCC 9689). The source organism is also available through the ATCC catalog.

In the parent strain, the presence of tcdA and tcdB genes was confirmed by PCR and the binary toxin gene cdtB was not amplified by PCR.

Handling information

Handling procedure
  1. Thaw the vial at room temperature and immediately place on ice. Avoid exposing the DNA to repeated freeze-thaw cycles as it may result in degradation.
  2. Gently mix the sample to ensure an even distribution of material.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
Aliquoting is highly recommended to avoid multiple freeze-thaws.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Depositors
ATCC
Cross references
GenBank X94328 C.difficile spo0A gene.
GenBank AX110003 Sequence 736 from Patent WO0123604.
GenBank AX110007 Sequence 740 from Patent WO0123604.
GenBank AX110095 Sequence 828 from Patent WO0123604.
GenBank AX111484 Sequence 2217 from Patent WO0123604.
GenBank AX111505 Sequence 2238 from Patent WO0123604.
GenBank AX111525 Sequence 2258 from Patent WO0123604.
GenBank U36481 Clostridium difficile sporulation protein homolog (spo0A) gene, partial cds.
GenBank S51968 {16S/23S ribosomal DNA spacer region} [Clostridium difficile, ATCC 9689, Genomic, 271 nt]
GenBank U43514 Clostridium difficile putative spoIVB (spoIVB) gene, complete cds, and phosphorylation-activated transcription factor spo0A (spo0a) gene, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Frequently Asked Questions

References

Curated Citations

Rolfe RD, Finegold SM. Purification and characterization of Clostridium difficile toxin. Infect. Immun. 25: 191-201, 1979. PubMed: 478634

Florin I, Thelestam M. Intoxication of cultured human lung fibroblasts with Clostridium difficile toxin. Infect. Immun. 33: 67-74, 1981. PubMed: 6167521

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

View All Curated Citations for this Product

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