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Bacillus spizizenii (Nakamura et al.) Dunlap et al.

6633

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Bacillus spizizenii strain NRS 231 is a whole-genome sequenced bacterium with applications as a test microorganism for USP <2021>, <61>, <71>, and <81>.
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Product category
Bacteria
Classification
Bacilli, Bacillales
Strain designation
NRS 231
Type strain
No
Genome sequenced strain
Yes
Former name
Bacillus subtilis subsp. spizizenii Nakamura et al.
Applications
Food testing
Media testing
Quality control
Pharmaceutical testing
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Produces restriction endonuclease Bsu6633I Shibata T, et al. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains. J. Bacteriol. 128: 473-476, 1976. PubMed: 824277 Ikawa S, et al. Genetic studies on site-specific endodeoxyribonucleases in Bacillus subtilis: multiple modification and restriction systems in transformants of Bacillus subtilis 168. Mol. Gen. Genet. 177: 359-368, 1980. PubMed: 6246395 Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885
Quality control strain
Sporicidal test Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity. London, UK:British Standards Institution;British Standard BS EN 12353:2006.  Chemical disinfectants --- Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants used in food, industrial, domestic and intitutional areas --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 13704:2002.  Chemical disinfectants and antiseptics --- Basic sporicidal activity --- Test method and requirements (phase 1, step 1). London, UK:British Standards Institution;British Standard BS EN 14347:2005.  Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution;British Standard BS EN 14885:2006.
Biotechnology
Food testing
Pharmaceutical and Personal Care
Assay of streptomycin European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23. Frerichs GN, Chandler MD. The identification and assay of antibiotics in live virus vaccines by electrophoresis in gel. J. Biol. Stand. 10: 205-211, 1982. PubMed: 6813333 Simon HJ, Yin EJ. Microbioassay of antimicrobial agents. Appl. Microbiol. 19: 573-579, 1970. PubMed: 4986725
Assay of streptonigrin Pittillo RF, Woolley C. Biological assay of streptonigrin (NSC 45383) in body fluids and tissues of mice. Antimicrob. Agents Chemother. 5: 82-85, 1974. PubMed: 4209070
Assay of tobramycin British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003 European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 Lightbown JW, Dixon H. The first international reference preparation of tobramycin. J. Biol. Stand. 10: 157-168, 1982. PubMed: 7096362
Assay of vancomycin European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Control strain Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A.  Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M26-A.  Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: carrier of agents for agar diffusion test. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-2.  Methods for the determination of susceptibility of pathogenic bacteria (without mycobacteria) to chemotherapeutic agents of discs for agar diffusion test example for getting the standard curve. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-2 Beiblatt 1.
Media testing British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003 European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (total viable aerobic count). Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.12, 1997 US Food & Drug Administration. GENERAL BIOLOGICAL PRODUCTS STANDARDS; General Provisions; Sterility Code of Federal Regulations Title 21: 21CFR610.12, Subpart B, 2005 U.S. Pharmacopeia Dietary Supplement Chapters: <2021> Microbial Enumeration Tests-Nutritional and Dietary Supplements. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011  Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.  Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.
Membrane filter testing Standard Test Method for Confirming the Sterility of Membrane Filters. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4196-05.
Assay of amoxicillin Lode H, et al. Comparative clinical pharmacology of three ampicillins and amoxicillin administered orally. J. Infect. Dis. 129: S156-S168, 1974. PubMed: 4834960
Assay of bekanamycin aminodeoxykanamycin, kanamycin B, kanendomycin
Assay of capreomycin sulfate capreomycin, capromycin British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003
Assay of colistimethate sodium U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of colistin colimycin U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of dactinomycin actinomycin, actinomycin D
Assay of dihydrostreptomycin sulfate dihydrostreptomycin European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 U.S. Pharmacopeia General Chapters: <71> STERILITY TESTS. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011 U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of erythromycin European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997
Assay of framycetin European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997
Assay of gentamicins gentamicin Frerichs GN, Chandler MD. The identification and assay of antibiotics in live virus vaccines by electrophoresis in gel. J. Biol. Stand. 10: 205-211, 1982. PubMed: 6813333
Assay of hygromycin B AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23.
Assay of kanamycin British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003 European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 Frerichs GN, Chandler MD. The identification and assay of antibiotics in live virus vaccines by electrophoresis in gel. J. Biol. Stand. 10: 205-211, 1982. PubMed: 6813333 Simon HJ, Yin EJ. Microbioassay of antimicrobial agents. Appl. Microbiol. 19: 573-579, 1970. PubMed: 4986725
Assay of lactam antibiotics beta-lactam antibiotics
Assay of lasalocid X-537A, lasalocid A
Assay of mitomycin mitomycin C
Assay of monensin AOAC International Monensin in feeds, microbiological method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 972.56. AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23.
Assay of neomycin European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 Frerichs GN, Chandler MD. The identification and assay of antibiotics in live virus vaccines by electrophoresis in gel. J. Biol. Stand. 10: 205-211, 1982. PubMed: 6813333 Simon HJ, Yin EJ. Microbioassay of antimicrobial agents. Appl. Microbiol. 19: 573-579, 1970. PubMed: 4986725
Assay of penicillin Frerichs GN, Chandler MD. The identification and assay of antibiotics in live virus vaccines by electrophoresis in gel. J. Biol. Stand. 10: 205-211, 1982. PubMed: 6813333
Assay of polymyxin B U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of spiramycin British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003 European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997
Sterility testing British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 2003 European Pharmacopoeia Commission Sterility. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.1, 1997 U.S. Pharmacopeia General Chapters: <71> STERILITY TESTS. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011 Bowman FW, et al. Collaborative study of aerobic media for sterility testing by membrane filtration. J. Pharm. Sci. 60: 1087-1088, 1971. PubMed: 5000479  Supplemental assay method for testing for preservative interference with sterility tests. :US Department of Agriculture;USDA, Center for Veterinary Biologics STSAM0903.01, 1999  Sterility test, Growth promotion test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.54.  Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14698-1:2003.
Susceptibility testing Medical microbiology - Susceptibility testing of pathogens to antimicrobial agents - Part 2: Active substance carriers for the agar diffusion test; Carrier loads and values required for drawing a standard curve. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-2 Beiblatt 2: 2002
Testing Microbial limit test. Tokyo, Japan:Japanese Pharmacopoeia;JP Jp14e.part I.35.  Microbial limit test for crude drugs. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.36.
Testing antibacterial activity Chemical disinfectants and antiseptics --- Surgical hand disinfection --- Test method and requirement (phase 2/step 2). London, UK:British Standards Institution;British Standard BS EN 12791:2005.
Testing bactericides
Validation control Cleanrooms and associated controlled environments --- Biocontamination control --- Part 1: General principles and methods. London, UK:British Standards Institution;British Standard BS EN ISO 14698:2003.
Growth promotion testing
Bioburden testing
Environmental monitoring
Microbial limit testing
Rabbit pyrogen testing
Bacterial endotoxin testing
Antibiotic assay
Potency testing
Recommended as a test microorganism for EP 2.6.1 Sterility
Recommended as a test microorganism for EP 2.6.12 Microbial Examination of Non-Sterile Products: Microbial Enumeration Tests
Recommended as a test microorganism for EP 2.6.27 Microbiological Control of Cellular Products
Recommended as a test microorganism for EP 2.6.31 Microbiological Examination of Herbal Medicinal Products for Oral Use
Recommended as a test microorganism for EP 2.7.2 Microbiological Assays of Antibiotics
Recommended as a test microorganism for EP Monographs
Recommended as a test microorganism for JP 16 4.05 I Microbiological Examination of Non-sterile Products: Total Viable Aerobic Count
Recommended as a test microorganism for JP 16 4.06 Sterility Test
Recommended as a test microorganism for JP 16 5.02 Microbial Limit Test for Crude Drugs
Recommended as a test microorganism for JP 16 Monographs
Recommended as a test microorganism for USP <2021> Microbial Enumeration Tests
Recommended as a test microorganism for USP <61> Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests
Recommended as a test microorganism for USP <71> Sterility Tests
Recommended as a test microorganism for USP <81> Antibiotics-Microbial Assays
Recommended as a test microorganism for AENOR UNE EN 1104: Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents

 

Preceptrol
No

Characteristics

Comments
Taxonomy
Enzymes

CA Dunlap et al. reclassified Bacillus subtilis subsp. spizizenii into the new species Bacillus spizizenii.  Some platforms and databases may still identify this species by its previous name.

Handling information

Medium
Temperature
30°C
Atmosphere
Aerobic
Incubation
Using a shaking incubator improves growth
Handling procedure
  1. Open vial.
  2. Using a single tube of #44 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #44 agar slant and/or plate.
  5. Incubate the tubes and plate at 30°C for 24 hours.
Handling notes

CA Dunlap et al. reclassified Bacillus subtilis subsp. spizizenii into the new species Bacillus spizizenii.  Some platforms and databases may still identify this species by its previous name.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Bacillus subtilis (Ehrenberg) Cohn
Depositors
NR Smith
Cross references
GenBank M38634 B.subitilis cellulase gene, 5' end, clone pBAG10.
GenBank J03767 B.subtilin spaS gene encoding subtilin, complete cds.
GenBank L07785 Bacillus subtilis spaR and spaK genes, complete coding regions.
GenBank M83944 Bacillus subtilis subtilin (spaD, spaB, spaC, spaS) genes, complete cds.
GenBank AB018486 Bacillus subtilis gene for 16S rRNA, partial sequence, strain:ATCC 6633.
GenBank AF004102 Bacillus subtilis strain ATCC 6633 thymidylate synthase (thyA) gene, partial cds.
GenBank J03294 Bacillus subtilis thioredoxin (trx), uvrB and aspartokinase II genes, complete cds.
GenBank L24075 Bacillus subtilis (ATCC 6633) spaB gene involved in subtilin biosynthesis, complete cds.
GenBank M99263 Bacillus subtilis operon encoding spaB, spaC, spaD, spaE and spaS genes, complete cds's; ORF, complete cds.
GenBank U38418 Bacillus subtilis chimeric proU operon, proV, proW, proX and proZ genes, complete cds, and spaE gene, partial cds.
GenBank U09819 Bacillus subtilis spaB, spaT, spaC, subtilin (spaS), spaI, spaF, spaE, spaG, spaR spaK and YvaN genes, complete cds.
GenBank M86869 Bacillus subtilis spaB gene, 3' end; spaT gene, complete cds; spaC gene, complete cds; subtilin (spaS) gene, putative cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Waleh NS, Ingraham JL. Pyrimidine ribonucleoside monophosphokinase and the mode of RNA turnover in Bacillus subtilis. Arch. Microbiol. 110: 49-54, 1976. PubMed: 189719

Shibata T, et al. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains. J. Bacteriol. 128: 473-476, 1976. PubMed: 824277

Ikawa S, et al. Genetic studies on site-specific endodeoxyribonucleases in Bacillus subtilis: multiple modification and restriction systems in transformants of Bacillus subtilis 168. Mol. Gen. Genet. 177: 359-368, 1980. PubMed: 6246395

The genus Bacillus, Agriculture Handbook 427. Washington, DC: ARS/USDA; 1973.

British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003

View All Curated Citations for this Product

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