Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux
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Cell Line Maintenance
Transferring the Cell Line
Storage and Culture Initiation of Toxoplasma
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
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Cole RA, et al. Biological and molecular characterizations of Toxoplasma gondii strains obtained from southern sea otters (Enhydra lutris nereis). J. Parasitol. 86: 526-530, 2000. PubMed: 10864250
L D Sibley, personal communication