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Chlorogonium elongatum Dangeard

50936

Product category
Protists
Product type
Algae
Classification
KINGDOM: Plantae
Type strain
No
Product format
Frozen
Storage conditions
-80°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Mission Collection Item
This is a Mission Collection Item

Documentation

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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

Handling information

Medium
Temperature
20-25°C
Culture system
Axenic
Handling procedure
Storage and Culture Initiation
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.  If liquid nitrogen storage facilities are not available, frozen ampules may be stored at or below -70°C for approximately one week.  Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C).  Storage of frozen material at this temperature will result in the death of the culture.
  1. To thaw a frozen ampule, place in a 35°C water bath, until thawed (2-3 min). Immerse the ampule just sufficiently to cover the frozen material.  Do not agitate the ampule.
  2. Immediately after thawing, aseptically transfer the entire contents to a single 16 x 125 mm screw-capped test tube containing 5 mL of ATCC Medium 5 broth (or alternatively, 5 mL ATCC Medium 351 supplemented with 0.1% Na Acetate).  Allow the tube to stand upright for one to several hours (or centrifuge very lightly for 3-5 min) to allow most cells to settle to the tube base.
  3. Gently remove most of the supernatant (save in a secondary tube), then resuspend the remaining cells in additional fresh medium to a total volume of 5-6 mL.
  4. Incubate tubes on a 15° horizontal slant with the cap screwed on loosely (loosened one half turn) at 20-25°C under a 14 hour light (~50 µEinsteins/m2/s irradiance)/10 hour dark cycle.
    Note: Some algae may grow poorly or not at all when recovered from the frozen state in broth media.  In such cases, the algae should initially be recovered on agar media before eventually transferring to broth culture.

Culture maintenance
  1. Inoculate a tube of fresh broth medium with 0.25 mL from a growing culture at or near peak density.
  2. Incubate tubes on a 15° horizontal slant with the cap screwed on loosely (loosened one half turn) at 20-25°C under a 14 hour light (~50 °Einsteins/m2/s irradiance)/10 hour dark cycle.
  3. Subculture as necessary (i.e., typically every 14-21 days in broth media).

History

Depositors
T Suzaki
Special collection
NSF - Protistology

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Sakaguchi M, et al. Involvement of a 40-kDa glycoprotein in food recognition, prey capture, and induction of phagocytosis in the protozoon Actinophrys sol. Eur. J. Protistol. 152: 33-41, 2001. PubMed: 11401035

Kinoshita E, et al. The ultrastructure of contractile tubules in the heliozoon Actinophrys sol and their possible involvement in rapid axopodial contraction. J. Eukaryot. Microbiol. 48: 519-526, 2001. PubMed: 11596916

Arikawa M, et al. Ca(2+)-dependent contractility of isolated and demembranated macronuclei in the hypotrichous ciliate Euplotes aediculatus. Cell Calcium 2003: 113-117, 2003. PubMed: 12531187

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