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Ectothiorhodospira mobilis (Oren et al.) Ventura et al.

49923

Product category
Bacteria
Product type
Extremophile
Strain designation
DSM 4180 [BN 9410, Oren EG-1]
Type strain
Yes; type strain of Ectothiorhodospira marismortui
Isolation source
Sulfur spring on Dead Sea shore
Geographical isolation
Israel
Product format
Frozen
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Biotechnology
Preceptrol
No

Characteristics

Extremophile type
Halophile
Phototroph
Sulfur oxidizer

Handling information

Medium
Temperature
35°C
Incubation
Under a tungsten lamp
Handling procedure
1.       Open vial according to enclosed instructions.

2.       This organism can tolerate brief exposure to oxygen, so it may be opened under aerobic conditions. Aseptically transfer 0.5 ml of medium #2010 to the vial and rehydrate the pellet.  Transfer this suspension back into a single, small tube (filled to capacity) of #2010 broth.  Plate 0.1 ml of the culture on any non-selective media and incubate aerobically in the dark at 26oC.  Seal the tube with a screw cap, and incubate at 26oC (room temperature) under a tungsten lamp.

3.       After four to seven days, growth should be evident as indicated by turbidity.  When examined microscopically, the cells appear as vibroid rods, in singles and pairs, some small chains.  Once growth has been detected, the culture should be transferred to fresh broth.  Subsequent growth should be detected within 48 to 72 hours.

4. For best results the medium should be pre-reduced, this can best be accomplished by:

a)       Steaming the medium then filling the test tube to capacity and sealing with a screw cap while the medium is still hot.

b)       Adding 5 to 6 drops of cysteine (3% stock concentration) or sodium sulfide (1.5% stock concentration) to each 5 to 6 ml of medium used.  After the reducing agent (cysteine or sodium sulfide) has been added fill the test tube to capacity and seal with a screw cap.  Let the test tube sit at room temperature for 30 minutes before inoculating.

Note that once growth has been established, the culture can be transferred without pre-reducing the medium by using a 10 to 20% inoculation.

History

Deposited as
Ectothiorhodospira marismortui Oren et al.
Depositors
DSM
Chain of custody
ATCC <-- DSM <-- A. Oren EG-1
Type of isolate
Environmental
Cross references
GenBank X93482 16S rDNA sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Validation list no. 32. Int. J. Syst. Bacteriol. 40: 105-106, 1990.

Oren A, et al. Ectothiorhodospira marismortui new species an obligately anaerobic moderately halophilic purple sulfur bacterium from a hypersaline sulfur spring on the shore of the Dead Sea. Arch. Microbiol. 151: 524-529, 1989.

Imhoff JF, Suling J. The phylogenetic relationship among Ectothiorhodospiraceae: a reevaluation of their taxonomy on the basis of 16S rDNA analyses. Arch. Microbiol. 165: 106-113, 1996. PubMed: 8593098

Ventura S, et al. Revision of species in the genus Ectothiorhodospira. Int. J. Syst. Evol. Microbiol. 50: 583-591, 2000. PubMed: 10758864

Ectothiorhodospira marismortui is recognized as a junior synonym of Ectothiorhodospira mobilis. ATCC 49923 is the type strain of Ectothiorhodospira marismortui.

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