Geobacillus thermoleovorans (Zarilla and Perry) Nazina et al.
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2. Using a single tube of #1554 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 55°C for 24 hours.
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Validation list no. 25. Int. J. Syst. Bacteriol. 38: 220-222, 1988.
Merkel GJ, Perry JJ. Phospholipid composition and heat sensitivity in a thermophilic bacterium. Biochim. Biophys. Acta 619: 68-75, 1980. PubMed: 7417470
Merkel GJ, Perry JJ. Effect of growth substrate on thermal death of thermophilic bacteria. Appl. Environ. Microbiol. 34: 626-629, 1977. PubMed: 596868
Zarilla KA, Perry JJ. Bacillus thermoleovorans, sp. nov., a species of obligately thermophilic hydrocarbon utilizing endospore-forming bacteria. Syst. Appl. Microbiol. 9: 258-264, 1987.
. . Adv. Aquat. Microbiol. 3: 109-139, 1985.