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Treponema denticola (ex Flügge) Chan et al.

35405

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Type strain. Produces methyl-accepting chemotaxis protein, major surface protein, and prolyl aminopeptidase.
Product category
Bacteria
Strain designation
a [CIP 103919, DSM 14222]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Periodontal pocket
Geographical isolation
Canada; Quebec; Montreal
Applications
Bioinformatics
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Produces major surface protein
Produces methyl-accepting chemotaxis protein
Produces prolyl aminopeptidase proline iminopeptidase
Preceptrol
No

Characteristics

Serotype
a
Comments
Benzoylarginine peptidase and iminopeptidase profiles
Genome sequenced strain

Handling information

Medium
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
  1. Open vial according to enclosed instructions.
  2. Under anaerobic conditions, aseptically withdraw approximately 0.5 to 1.0 mL from a tube of #1494 broth.  Rehydrate the entire pellet.
  3. Transfer this aliquot back into the broth tube.
  4. Additional tubes of broth may be inoculated with 0.5 mL each of the suspension.  You may also use 0.5 mL to inoculate a #1494 slant.  Inoculate two Tryptic Soy Agar plates with 5% sheep blood (ATCC Medium #260).  Incubate one blood plate anaerobically to check for colony formation and incubate the second plate aerobically to check for purity.
  5. Incubate tubes and plate under anaerobic conditions at 37°C.  Incubate the second blood plate aerobically. 
  6. After 3-6 days, growth should be evident by slight turbidity in the broth and flocculent sediment.  To observe growth, examine a wet mount of the broth under phase microscopy.  Colonies of agar may take longer to appear.  No growth should occur on the blood agar plate incubated aerobically.

 

ANAEROBIC CONDITIONS:   

Anaerobic conditions for transfer may be obtained by either of the following:

  • Use of an anaerobic gas chamber, or                                        
  • Placement of test tubes under a gassing cannula system hooked to anaerobic gas.   

 

Anaerobic conditions for incubation may be obtained by any of the following:

  • Loose screw caps on test tubes in anaerobic chamber,                       
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar, or   
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.   

Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Treponema denticola (Brumpt) Chen
Depositors
E Chan
Type of isolate
Human
Cross references
GenBank U33210 dmcA
GenBank L36851 Treponema denticola fliG gene, complete cds.
GenBank L25421 Treponema denticola phosphatase gene, 5' end.
GenBank AF082511 Treponema denticola mgl operon, partial sequence.
GenBank U29399 Treponema denticola major outer sheath protein (msp) gene, complete cds.
GenBank L25603 Treponema denticola (ATCC 35405) chymotrypsin-like protease gene, complete cds.
GenBank U84257 Treponema denticola methyl-accepting chemotaxis protein B (dmcB) gene, partial cds.
GenBank AF012922 Treponema denticola methyl-accepting chemotaxis protein (mcpA) gene, complete cds.
GenBank AF042861 Treponema denticola OppA (oppA), putative OppB (oppB), putative OppC (oppC), putative OppD (oppD), and putative OppF (oppF) genes, complete cds.
GenBank AF232219 Treponema denticola putative alcohol dehydrogenase, FliQ (fliQ), FliR (fliR), and FlhB (flhB) genes, complete cds; and FlhA (flhA) gene, partial cds.
GenBank U78776 Treponema denticola gufa gene, partial cds, putative flagellar operon flgB, flgC, fliE, fliF, fliG, fliH, fliI and fliJ genes, complete cds, and fdgA gene, partial cds.
GenBank AF122909 Treponema denticola Tap1 (tap1), flagellar hook assembly scaffolding protein FlgD (flgD), flagellar hook protein FlgE (flgE), flagellar motor protein MotA (motA), flagellar motor protein MotB (motB), FliL (fliL), flagellar switch protein FliM
GenBank AF139203 Treponema denticola strain ATCC 35405 16S ribosomal RNA gene, partial sequence
GenBank AE017226 Treponema denticola ATCC 35405, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Makinen KK, et al. Dominance of iminopeptidase activity in the human oral bacterium Treponema denticola ATCC 35405. Curr. Microbiol. 14: 341-346, 1987.

Makinen KK, et al. Benzoylarginine peptidase and iminopeptidase profiles of Treponema denticola strains isolated from the human periodontal pocket. Curr. Microbiol. 14: 85-89, 1986.

Makinen KK, et al. Proline iminopeptidase from the outer cell envelope of the human oral spirochete Treponema denticola ATCC 35405. Infect. Immun. 64: 702-708, 1996. PubMed: 8641769

Chan EC, et al. Treponema denticola (ex Brumpt 1925) sp. nov., nom. rev., and identification of new spirochete isolates from periodontal pockets. Int. J. Syst. Bacteriol. 43: 196-203, 1993. PubMed: 8494734

Cheng SL, et al. Comparative study of six random oral spirochete isolates. Serological heterogeneity of Treponema denticola. J. Periodontal Res. 20: 602-612, 1985. PubMed: 2935615

View All Curated Citations for this Product

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