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Legionella pneumophila subsp. pneumophila Brenner et al.


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Legionella pneumophila subsp. pneumophila strain Philadelphia-1 was isolated from a human lung. This whole-genome sequenced bacterial type strain can be used in media testing, emerging infectious disease research, and respiratory disease research.
Product category
Product type
Preceptrol culture
Strain designation
Type strain
Genome sequenced strain
Isolation source
Infectious disease research
Media testing
Respiratory disease research
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Media testing
Emerging infectious disease research
Respiratory research


Genome sequenced strain
Fluorescent antibody serogroup 1
Fatty acid composition

Handling information

95% Air, 5% CO2
Handling procedure
  1. Open vial according to enclosed instructions or visit for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #1099 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1099 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
  3. Use several drops of the primary broth tube to inoculate a #1099 plate and/or #1099 agar slant.
  4. Incubate at 37°C for 48-72 hours in an atmosphere of 5% CO2.
Handling notes
Legionella species are nutritionally fastidious and are inhibited by media of poor caliber, so strict attention should be paid to the quality of the ingredients.  The pH of the medium should be checked when cool, and is most critical.  Unbuffered medium should not be used.  Exposure of medium to light, particularly when hot, may also result in the accumulation of peroxides which can be inhibitory to the bacteria. 

Before rehydration, vials may be stored at +2 to +8°C for extended periods with minimal loss of viability.  Subculturing to maintain culture is discouraged because of the possibility of mutation, selection of variants, or contamination.  To minimize changes, it is recommended that cells be harvested and stored at -70°C or below.

Purified genomic DNA of this strain is available (ATCC 33152D-5 and ATCC 33152DQ).

Additional information on this culture is available on the ATCC web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Legionella pneumophila subsp. pneumophila Brenner et al.
RE Weaver
Chain of custody
ATCC <-- RE Weaver <-- JE McDade
Type of isolate
Cross references
GenBank X72354 L.pneumophila Sgp.1 (ATCC 33152) gene for 5S ribosomal RNA.
GenBank U68079 Legionella pneumophila coded portion of proteolysis tag tmRNA gene.
GenBank AX109589 Sequence 322 from Patent WO0123604.
GenBank AX111039 Sequence 1772 from Patent WO0123604.
GenBank M59157 Legionella pneumophila 16S ribosomal RNA.
GenBank Z24705 L.pneumophila 23S and 5S rRNA genes, partial CDS's.
GenBank AJ011775 Legionella pneumophila djlA and kdtA (waaA) genes.
GenBank M19444 L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 2 of 3.
GenBank M19450 L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 1 of 3.
GenBank M22266 L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 3 of 3.
GenBank Z30431 L.pneumophila Serogroup 1 Philadelphia-1 gene for 23S ribosomal RNA and 5S ribosomal RNA.
GenBank AF095231 Legionella pneumophila strain ATCC 33152 defect in organelle trafficking protein (dotA) gene, partial cds.
GenBank AE017354 Legionella pneumophila subsp. pneumophila str. Philadelphia 1, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Validation list no. 3. Int. J. Syst. Bacteriol. 29: 436, 1979.

Validation list no. 29. Int. J. Syst. Bacteriol. 39: 205-206, 1989.

Brenner DJ, et al. Classification of the Legionnaires' disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann. Intern. Med. 90: 656-658, 1979. PubMed: 434652

Moss CW, et al. Cellular fatty acid composition of isolates from Legionnaires disease. J. Clin. Microbiol. 6: 140-143, 1977. PubMed: 893658

Brenner DJ, et al. Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov.. J. Clin. Microbiol. 26: 1695-1703, 1988. PubMed: 3053773

View All Curated Citations for this Product

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