Prevotella disiens (Holdeman and Johnson) Shah and Collins
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2. Using a single tube of #1589 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1589 agar slant and/or plate.
5. Incubate the tubes and plate at 37oC for 24-48 hours.
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Holdeman LV, Johnson JL. Bacteroides disiens sp. nov. and Bacteroides bivius sp. nov. from human clinical infections. Int. J. Syst. Bacteriol. 27: 337-345, 1977.
Shah HN, Collins DM. Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int. J. Syst. Bacteriol. 40: 205-208, 1990. PubMed: 2223612
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.