Porphyromonas levii (Johnson and Holdeman) Shah et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 37°C. Incubate blood plate aerobically at 37°C.
5. Within 48 hours, growth should be evident by turbidity in the broth. Colonies are entire, glistening, circular, smooth, smaller, and pinpoints. No growth should occur on the blood agar plate incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Johnson JL, Holdeman LV. Bacteroides intermedius comb. nov. and descriptions of Bacteroides corporis sp. nov. and Bacteroides levii sp. nov.. Int. J. Syst. Bacteriol. 33: 15-25, 1983.
Shah HN, et al. Reclassification of Bacteroides levii (Holdeman, Cato, and Moore) in the genus Porphyromonas, as Porphyromonas levii comb. nov.. Int. J. Syst. Bacteriol. 45: 586-588, 1995.
Lev M, et al. Succinate as a growth factor for Bacteroides melaninogenicus. J. Bacteriol. 108: 175-178, 1971. PubMed: 4941554
Lev M, Milford AF. Sensitivity of a Bacteroides melaninogenicus strain to monosaccharides: effect on enzyme induction. J. Bacteriol. 121: 152-159, 1975. PubMed: 1116985
Lev M. Vitamin K deficiency in Fusiformis nigrescens. I. Influence on whole cells and cell envelope characteristics. J. Bacteriol. 95: 2317-2324, 1968. PubMed: 5669903