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Pseudomonas aeruginosa (Schroeter) Migula

27853

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This bacterial strain has applications in media and susceptibility testing, evaluation of Mueller-Hinton Agar, and as a quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, Micro-Media, MicroScan™, Roche Diagnostics, and Sensititre products.
Product category
Bacteria
Product type
Preceptrol culture
Strain designation
Boston 41501
Type strain
No
Isolation source
Blood culture
Applications
Bioinformatics
Food testing
Media testing
Quality control
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Control strain
Food testing
Opportunistic pathogen research
Evaluation of Mueller-Hinton agar Ref Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2. RefUS General Services Administration Mueller Hinton Medium, dehydrated. Washington, DC:US General Services Administration;Commercial Item Description A-A-50904A, 1986
Media testing Ref Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. Ref Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003. Ref Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003 Ref Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.
Quality control strain Ref API 20 NE. bioMerieux. Ref BD Enterotube II QC Set. BD Becton Dickinson; Enterotube  Ref BD Oxi/Ferm II QC Set. BD Becton Dickinson; Oxi/Ferm  RefBD Phoenix Gram Negative AST NMIC/ID QC Set. BD Becton Dickinson; Phoenix  Ref MicroScan Dried Gram Negative Panel QC Set. Dade/Siemens. Ref Sensititre™ Automated Gram Positive ID. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems. Ref Sensititre™ MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems. ref
Susceptibility disc testing Ref Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. Ref Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A. Ref Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A.
Susceptibility testing Ref Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8. Ref Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3. Ref Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007 Ref Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P. RefMedical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-3: 1989 Ref Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-7: 1994, 1994 Ref Medical microbiology: methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents; determination of MICs by agar dilution method. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-6: 1989, 1989 RefMedical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002 Ref Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006.
Testing Ref Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin (ADA-V). Washington, DC:Environmental Protection Agency;EPA EPA Method 1605, 2001
Quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, Micro-Media, MicroScan®, Roche Diagnostics, Sensititre products, and the Etest Metallo Beta-Lactamase (MBL) strip.
Preceptrol
Yes

Characteristics

Comments
Incorrectly cited in previous editions of this catalog as identical to NCTC 10662.
effect of salt concentration on gentamicin susceptibility
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
  5. Incubate the tubes and plate at 37°C for 24 hours.
Handling notes

Two colony types may be observed.  They have been individually characterized and found to be identical.  The predominant type is erose, rough, low convex, and approximately 2 mm in diameter.  The secondary type is entire, glistening, smooth, and small.  This strain produces both fluorescein and pyocyanin pigments.  Bacteriophage may be present in the culture.

To prevent the proliferation of the second colony type, avoid passing this strain through broth for transfers.  It is recommended to wash a slant with phosphate buffer and use that to inoculate further agar plates and/or agar flasks if needed.

 

Purified genomic DNA of this strain is available as ATCC® 27853D-5™. Certified Reference Material of this strain is available as ATCC® CRM-27853™.

 

Several Genbank accessions are available for this item:

  1. Nucleotide (GenBank) : AB037545 Pseudomonas aeruginosa gene for 16S ribosomal RNA, strain:ATCC 27853.
  2. Nucleotide (GenBank) : AF094719 Pseudomonas aeruginosa strain ATCC 27853 16S ribosomal RNA gene, partial sequence.
  3. Nucleotide (GenBank) : M63957 Pseudomonas aeruginosa heat shock protein (groES and groEL) genes, complete cds.
  4. Nucleotide (GenBank) : U07832 Pseudomonas aeruginosa helicase I (helA) gene, partial cds.
  5. Nucleotide (GenBank) : U64101 Pseudomonas aeruginosa heat shock protein (groesEL) gene, partial cds.
  6. Nucleotide (GenBank) : U67855 Pseudomonas aeruginosa UDP-3-O-acyl-GlcNAc deacetylase (lpxC) gene, complete cds.
  7. Nucleotide (GenBank) : X65933 P. aeruginosa pa-1L gene for PA-I galactophilic lectin.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Pseudomonas aeruginosa (Schroeter) Migula
Depositors
AA Medeiros
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Cross references
GenBank X65933 P. aeruginosa pa-1L gene for PA-I galactophilic lectin.
GenBank U07832 Pseudomonas aeruginosa helicase I (helA) gene, partial cds.
GenBank U64101 Pseudomonas aeruginosa heat shock protein (groesEL) gene, partial cds.
GenBank AB037545 Pseudomonas aeruginosa gene for 16S ribosomal RNA, strain:ATCC 27853.
GenBank M63957 Pseudomonas aeruginosa heat shock protein (groES and groEL) genes, complete cds.
GenBank U67855 Pseudomonas aeruginosa UDP-3-O-acyl-GlcNAc deacetylase (lpxC) gene, complete cds.
GenBank AF094719 Pseudomonas aeruginosa strain ATCC 27853 16S ribosomal RNA gene, partial sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Medeiros AA, et al. Effect of salt concentration on the apparent in-vitro susceptibility of Pseudomonas and other gram-negative bacilli to gentamicin. J. Infect. Dis. 124: 59-66, 1971. PubMed: 5001630

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A.

View All Curated Citations for this Product

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