Bifidobacterium catenulatum Scardovi and Crociani
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2. Under anaerobic conditions, withdraw 0.5 ml of #1053 from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional tubes may be inoculated with 0.5 ml each from the suspension and 0.2 ml for slants. Streak several blood plates to check for colonial morphology and purity.
4. Incubate tubes under anaerobic conditions at 37oC. Incubate one plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
5. After 1‑2 days, growth is evident by good turbidity in the broth and colonies on the agar slant. The aerobic plate may have a thin film in the area of heaviest inoculation, however it should show no other signs of aerobic contamination.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Colonies after 24 hours are pinpoint, circular, entire, convex, smooth, glistening, and translucent.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Scardovi V, Crociani F. Bifidobacterium catenulatum, Bifidobacterium dentium, and Bifidobacterium angulatum: Three new species and their deoxyribonucleic acid homology relationships. Int. J. Syst. Bacteriol. 24: 6-20, 1974.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.