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Salmonella enterica subsp. enterica (ex Kauffmann and Edwards) Le Minor and Popoff serovar Typhimurium

14028

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Salmonella enterica subsp. enterica serovar Typhimurium strain CDC 6516-60 is a whole-genome sequenced bacterium that was isolated from the pooled heart and liver tissue of four-week-old chickens. This bacterium has applications in enteric disease research, media testing, and water testing.        
Product category
Bacteria
Product type
Preceptrol culture
Strain designation
CDC 6516-60
Type strain
No
Isolation source
Tissue from pools of heart and liver from 4-week-old chickens
Applications
Bioinformatics
Enteric disease research
Food testing
Infectious disease research
Media testing
Water testing
Quality control
Pharmaceutical testing
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Control Culture
Media testing
Preparatory test control
Enteric Research
Emerging infectious disease research
Pharmaceutical and Personal Care
Water testing
Recommended as a test microorganism for EP 2.6.13 Microbial Examination of Non-sterile Products: Test for Specified Micro-organisms
Recommended as a test microorganism for EP 2.6.31 Microbiological Examination of Herbal Medicinal Products for Oral Use
Recommended as a test microorganism for JP 16 4.05 II Microbiological Examination of Non-sterile Products: Tests for Specified Micro-organisms
Recommended as a test microorganism for USP <62> Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms
Preceptrol
Yes

Characteristics

Serotype
I 4,5,12:i:1,2 (Determined by depositor)
Comments
This strain is recommended by ATCC for use in the tests described in U.S. Pharmacopeia, 23rd rev., <2021>, p. 2180, 1995, where only the taxon is specified.
The depositor states that this strain possesses these antigenic properties: 4,5,12:i:1,2.
Two colony types can be distinguished on sheep blood agar. One colony type reacts with Group B antisera; the other does not.

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
  5. Incubate all tubes and plate at 37°C for 24 hours.
Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Salmonella typhimurium (Loeffler) Castellani and Chalmers
Depositors
CDC
Chain of custody
CDC <-- University of Missouri
Type of isolate
Animal
Cross references
GenBank AX109618 Sequence 351 from Patent WO0123604.
GenBank AX111148 Sequence 1881 from Patent WO0123604.
GenBank AX111476 Sequence 2209 from Patent WO0123604.
GenBank U67869 Salmonella typhimurium SirA gene, complete cds.
GenBank U06133 Salmonella typhimurium ATCC 14028 isolate MS1 Tn10 insertion site.
GenBank U06126 Salmonella typhimurium ATCC 14028 isolate MS1321 Tn10 insertion site.
GenBank U06127 Salmonella typhimurium ATCC 14028 isolate MS1413 Tn10 insertion site.
GenBank U06128 Salmonella typhimurium ATCC 14028 isolate MS1523 Tn10 insertion site.
GenBank U06129 Salmonella typhimurium ATCC 14028 isolate MS1521 Tn10 insertion site.
GenBank U06130 Salmonella typhimurium ATCC 14028 isolate MS1633 Tn10 insertion site.
GenBank U06131 Salmonella typhimurium ATCC 14028 isolate MS1668 Tn10 insertion site.
GenBank U06132 Salmonella typhimurium ATCC 14028 isolate MS3493 Tn10 insertion site.
GenBank U06134 Salmonella typhimurium ATCC 14028 isolate MS2097 Tn10 insertion site.
GenBank U06135 Salmonella typhimurium ATCC 14028 isolate MS2101 Tn10 insertion site.
GenBank U06136 Salmonella typhimurium ATCC 14028 isolate MS4290 Tn10 insertion site.
GenBank U06137 Salmonella typhimurium ATCC 14028 isolate MS3747 Tn10 insertion site.
GenBank U06138 Salmonella typhimurium ATCC 14028 isolate MS5076 Tn10 insertion site.
GenBank U06139 Salmonella typhimurium ATCC 14028 isolate MS7481 Tn10 insertion site.
GenBank U06140 Salmonella typhimurium ATCC 14028 isolate MS6290 Tn10 insertion site.
GenBank U06141 Salmonella typhimurium ATCC 14028 isolate MS9020 Tn10 insertion site.
GenBank U06142 Salmonella typhimurium ATCC 14028 isolate MS8282 Tn10 insertion site.
GenBank U31849 Salmonella typhimurium pagD, envE, msgA and envF genes, complete cds.
GenBank U65941 Salmonella typhimurium O-antigen acetylase (oafA) gene, complete cds.
GenBank AF129435 Salmonella typhimurium bovine colonization factor BcfC gene, complete cds.
GenBank AF130422 Salmonella typhimurium bovine colonization factor operon, complete sequence.
GenBank AF175343 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch1c, genomic survey sequence
GenBank AF175344 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch6c, genomic survey sequence
GenBank AF175345 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch8c, genomic survey sequence
GenBank AF175357 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh2, genomic survey sequence
GenBank AF175358 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh3, genomic survey sequence
GenBank AF175359 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh4, genomic survey sequence
GenBank AF175360 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh6, genomic survey sequence
GenBank AF175361 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh8, genomic survey sequence
GenBank AF175362 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh9, genomic survey sequence
GenBank AF175346 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch14c, genomic survey sequence
GenBank AF175347 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch16c, genomic survey sequence
GenBank AF175348 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch20c, genomic survey sequence
GenBank AF175349 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch26c, genomic survey sequence
GenBank AF175350 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch36c, genomic survey sequence
GenBank AF175351 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch37c, genomic survey sequence
GenBank AF175352 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch39c, genomic survey sequence
GenBank AF175353 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch43c, genomic survey sequence
GenBank AF175354 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch44c, genomic survey sequence
GenBank AF175355 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch45c, genomic survey sequence
GenBank AF175356 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone ch46c, genomic survey sequence
GenBank AF175363 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh13, genomic survey sequence
GenBank AF175364 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh14, genomic survey sequence
GenBank AF175365 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh15, genomic survey sequence
GenBank AF175366 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh16, genomic survey sequence
GenBank AF175367 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh17, genomic survey sequence
GenBank AF175368 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh19, genomic survey sequence
GenBank AF175369 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh22, genomic survey sequence
GenBank AF175370 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh25, genomic survey sequence
GenBank AF175371 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh26, genomic survey sequence
GenBank AF175372 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh27, genomic survey sequence
GenBank AF175373 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh29, genomic survey sequence
GenBank AF175374 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh30, genomic survey sequence
GenBank AF175375 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh31, genomic survey sequence
GenBank AF175376 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh32, genomic survey sequence
GenBank AF175377 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh34, genomic survey sequence
GenBank AF175378 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh36, genomic survey sequence
GenBank AF175379 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh37, genomic survey sequence
GenBank AF175380 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh38, genomic survey sequence
GenBank AF175381 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh39, genomic survey sequence
GenBank AF175382 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh41, genomic survey sequence
GenBank AF175383 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh45, genomic survey sequence
GenBank AF175384 Salmonella typhimurium ATCC 14028 Salmonella typhimurium genomic clone cjh50, genomic survey sequence
GenBank U18559 Salmonella typhimurium long polar fimbriae proteins (lpfA, lpfB, lpfC, lpfD, lpfE) genes, complete cds and (orf1, orf2) genes, partial cds.
GenBank U21676 Salmonella typhimurium PrgH protein (prgH), PrgI protein (prgI), PrgJ protein (prgJ), and PrgK protein (prgK) genes complete cds, and OrgA protein (orgA) partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. We cannot ship this item until we receive this permit.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

Judicial Commission of the Internat. The type species of the genus Salmonella Lignieres 1900 is Salmonella enterica (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with the type strain LT2T, and conservation of the epithet enterica in Salmonella enterica over all earlier epithets that may be applied to this species. Opinion 80. Int J Syst Evol Microbiol 55: 519-520, 2005. PubMed: 15653929

Tindall BJ, et al. Nomenclature and taxonomy of the genus Salmonella. Int J Syst Evol Microbiol 55: 521-524, 2005. PubMed: 15653930

Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1682, 2005

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

View All Curated Citations for this Product

Frequently Asked Questions

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