• Quick Order

Pseudomonas putida (Trevisan) Migula


Download Genome Learn about our Enhanced Authentication Initiative
Pseudomonas putida [A.3.12, ATCC 23467, NCIB 9494, NCTC 10936, R.Y. Stanier 90] is a whole-genome sequenced bacterial type strain that degrades aromatic acids. This culture can be used as a bacteriophage host and as prey for Peredibacter starrii (ATCC 15145).
Product category
Product type
Preceptrol culture
Strain designation
[A.3.12, ATCC 23467, NCIB 9494, NCTC 10936, R.Y. Stanier 90]
Type strain
Genome sequenced strain
Product format
Storage conditions
2°C to 8°C
Buy Now
Price: $72.00 EA
Discounts may be available for our fellow nonprofit organizations.

Generally ships within 1-3 business days


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Catabolizes mandelate
Degrades aromatic acids
Hydrolyzes d,L-alpha-amino acid amides
Metabolizes 2-ketogluconate 2-ketogluconic acid
Metabolizes beta-ketoadipate
Bacteriophage host


Antigenic properties
Biotype A
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
  5. Incubate all tubes and plate at 26°C for 24 hours.
Handling notes
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Pseudomonas fluorescens Migula
AB Pardee
Chain of custody
ATCC <-- AB Pardee <-- RY Stanier A.3.12
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Cross references
GenBank AJ010261 Pseudomonas putida pepA gene.
GenBank M19043 P.putida mandelate racemase gene, complete cds.
GenBank M33923 Pseudomonas putida urocanase (hutU) gene, complete cds.
GenBank M35140 P.putida histidine ammonia-lyase (hutH) gene, complete cds.
GenBank L33795 Pseudomonas putida regulatory protein (pcaR) gene, complete cds.
GenBank U74323 Pseudomonas putida glucose-binding protein (gltB) gene, complete cds.
GenBank AF094736 Pseudomonas putida strain ATCC 12633 16S ribosomal RNA gene, partial sequence.
GenBank M33922 P.putida histidine utilization genes repressor protein (hut) gene, complete cds.
GenBank M69159 Pseudomonas putida alpha-ketoglutarate semialdehyde dehydrogenase gene, complete cds.
GenBank AB039451 Pseudomonas putida gyrB gene for DNA gyrase subunit B, partial cds, strain:ATCC 12633.
GenBank D37923 Pseudomonas putida (strain IFO 14164 (ATCC 12633)) gene for 16S ribosomal RNA, partial sequence.
GenBank D86020 Pseudomonas putida rpoD gene for RNA polymerase sigma-70 factor, partial cds, strain: IFO 14164.
GenBank AB039581 Pseudomonas putida rpoD gene for DNA-directed RNA polymerase subunit D, partial cds, strain:ATCC 12633.
GenBank J05293 Pseudomonas putida benzoylformate decarboxylase (mdlC), S-mandelate dehydrogenase (mdlB), and mandelate racemase (mdlA) genes, complete cds.
GenBank AF032970 Pseudomonas putida inducible histidine transporter (hutT), imidazolone propionate hydrolase (hutI), and N-formylglutamate amidohydrolase (hutG) genes, complete cds; and proline iminopeptidase (pip1) gene, partial cds.
GenBank AF183382 Pseudomonas putida flagellar protein FlhA (flhA) gene, partial cds; flagellar protein FlhF (flhF), MinD homolog, motility sigma factor FliA (fliA), response regulator CheY (cheY), dephosphorylase CheZ (cheZ), sensor histidine kinase CheA (cheA),

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Stanier RY. Acetic acid production from ethanol by fluorescent pseudomonads. J. Bacteriol. 54: 191-194, 1947.

Stanier RY. Simultaneous adaptation: a new technique for the study of metabolic pathways. J. Bacteriol. 54: 339-348, 1947.

Hegeman GD, Root RT. The effect of a non-metabolizable analog on mandelate catabolism in Pseudomonas putida. Arch. Microbiol. 110: 19-25, 1976. PubMed: 1015936

Torrontegui D, et al. The uptake of 2-ketogluconate by Pseudomonas putida. Arch. Microbiol. 110: 43-48, 1976. PubMed: 1015939

Kamphuis J, et al. New developments in the synthesis of natural and unnatural amino acids. Ann. N.Y. Acad. Sci. 672: 510-527, 1992. PubMed: 1476387

View All Curated Citations for this Product

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.



US and Puerto Rico


Outside the US


Hours of Operation

9:30am - 5:30pm
US Eastern Time