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Bacillus pacificus Delaporte


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Bacillus pacificus strain NRS 248 is a xylose-positive variant. This bacterium is whole-genome sequenced and is a microbiome standard component.

Product category
Strain designation
NRS 248
Type strain
Genome sequenced strain
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information




Xylose-positive variant.
Genome sequenced strain.
Microbiome standard component. Component of MSA-1000, MSA-1001, MSA-1002, MSA-1003, MSA-2002, and MSA-2003.

Handling information

Handling procedure
  1. Open vial according to enclosed instructions or visit for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #3 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #3 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #3 plate and/or #3 agar slant.
  4. Incubate at 30°C for 24-48 hours.
Handling notes
Purified genomic DNA of this strain is available as ATCC® 10987D-5™.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Bacillus cereus Frankland and Frankland
NR Smith
Chain of custody
ATCC <-- NR Smith <-- Herron
Cross references
GenBank Y08953 B.cereus kcy gene.
GenBank X62374 B.cereus mreb gene.
GenBank Y09253 B.cereus dedA gene.
GenBank Y10981 B.cereus proA gene.
GenBank Y11217 B.cereus addA gene.
GenBank Y11218 B.cereus sp5k gene.
GenBank Y11171 B.cereus bc542a gene.
GenBank Y09254 B.cereus orf bc87, partial.
GenBank AJ010140 Bacillus cereus alkC gene.
GenBank X98455 B.cereus orf1 and snf2 gene.
GenBank Y09252 B.cereus pur9 gene, partial.
GenBank Y09255 B.cereus dnaI gene, partial.
GenBank Y10909 B.cereus sp5k gene, partial.
GenBank Y11141 B.cereus colA gene, partial.
GenBank Y11172 B.cereus appC gene, partial.
GenBank Y10908 B.cereus cysA and wapA genes.
GenBank Y11170 B.cereus bc541a gene, partial.
GenBank Y09719 B.cereus bct1 gene and glr gene.
GenBank Y11305 Bacillus cereus glpT gene, partial.
GenBank AJ010130 Bacillus cereus hom gene, partial.
GenBank AJ010129 Bacillus cereus yxaA gene, partial.
GenBank AJ010133 Bacillus cereus yvgN gene, partial.
GenBank AJ010134 Bacillus cereus bc8a gene, partial.
GenBank AJ010136 Bacillus cereus ytiP gene, partial.
GenBank AJ010137 Bacillus cereus ydhE gene, partial.
GenBank AJ010331 Bacillus cereus bla1 gene, partial.
GenBank AJ010132 Bacillus cereus bc89a gene, partial.
GenBank Y11140 B.cereus bc48a and bc48b partial genes.
GenBank AJ010135 Bacillus cereus partial rpsB, tsf genes.
GenBank Y09323 B.cereus DNA, two genes with unknown function.
GenBank Y11138 B.cereus DNA for ORF1, ORF2 and ORF3 (2402 bp).
GenBank Y11139 B.cereus DNA for ORF1, ORF2 and ORF3 (2994 bp).
GenBank Y09433 B.cereus rs1h gene for 30S ribosomal protein S1.
GenBank Y09212 B.cereus partial gltT, aspA and partial maoX genes.
GenBank X60713 B.cereus gene for restriction endonuclease type III.
GenBank Y09322 B.cereus nupC gene (partial) and lytR gene (partial).
GenBank AJ010139 Bacillus cereus yfjS, alk1 genes and partial fumA gene.
GenBank AJ010111 Bacillus cereus pycA, ctaA, ctaB and ctaC genes, partial.
GenBank AJ010128 Bacillus cereus bc297a, alkD genes and partial glyS gene.
GenBank AJ010131 Bacillus cereus yfkH gene and partial ykvW, bc333c genes.
GenBank AJ010138 Bacillus cereus spoIIID, mbl genes and partial spoIIQ, ywpB genes.
GenBank AJ272331 Bacillus cereus cheA gene for histidine kinase, strain ATCC 10987.
GenBank AJ000394 Bacillus cereus celR (partial), orf2, chrA, ydjC, celF (partial) genes.
GenBank AJ007510 Bacillus cereus mcrB (partial), t3mod and t3res genes, and 210b, 210e and 210f.
GenBank AJ007788 Bacillus cereus ilvD (partial), ilvA, capA, bc299d, bc299e and bc299f genes (partial).
GenBank AJ272332 Bacillus cereus fliY gene for component of the flagellar switch complex, strain ATCC 10987.
GenBank AE017194 Bacillus cereus ATCC 10987, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Smith NR, et al. Aerobic spore forming bacteria. US Dep Agric Monogr 16: 1-148, 1952.

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