Solute transporter carrier (SLC)-expressing cells play an important role in the absorption, distribution, and clearance of a wide variety of drugs from the kidney and other organs.
Researchers need models of in vitro kidney transporters that have normal human kidney origin, functioning transporters, clinical predictability, and consistent data output for drug interaction studies. Unfortunately, primary renal epithelial cells lose OAT1, OCT2, and OAT3 transporter expression quickly in culture.
ATCC has been able to utilize hTERT-immortalized primary renal proximal tubule epithelial cells to create kidney transporter cells that stably overexpress either the OAT1, OCT2, or OAT3 gene for drug toxicity screening and more.
In addition to renal SLC-expressing cells, ATCC has accessioned many cell lines that possess knockouts in SLC transporters such as SLC25, MITCH1 and SLC35. These cell lines were derived from parental cells such as HCT116 and can be used to identity roles for these understudied proteins.
Renal Solute Carrier Uptake Models
Learn more about the creation and application of hTERT-immortalized human primary RPTECs that stably express the OAT1, OCT2, or OAT3 proteins.
Reliable Tools for Assessing Renal Solute Passage and Drug Toxicity
Watch this webinar to learn how hTERT-immortalized primary renal epithelial cells can be incorporated into your toxicity testing workflow.