pENTR_L1L2_BM3R1-VP64 (ATCC® SB-1095)

Permits and Restrictions

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Designations pENTR_L1L2_BM3R1-VP64
Applications A TetR homolog-based synthetic activator for gene expression regulation
Vector Total size of construct (kb): 3.390
Vector type: Entry vector
Markers: kanR
Insert Insert size (Kb): 0.78
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
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Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

This construct contains a fusion of the TetR homolog BM3R1 and a VP64 activation domain.

Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737

Ron Weiss, personal communication