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pCarrier 7 (426) (ATCC® SB-1024)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

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Designations pCarrier 7 (426)
Depositors R Weiss, Massachusetts Institute of Technology
Applications A carrier vector in the ATCC® Synthetic Biology Yeast Tool Kit designed for use in the construction of a single transcription unit and gene circuit.
Vector Total size of construct (kb): 5.922
Vector type: High-copy (2 µ) yeast vector
Markers: ampR (E. coli)
Preparation
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Related Products ATCC SB-1004
ATCC SB-1005
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
This plasmid is a Saccharomyces cerevisiae/Escherichia coli shuttle vector. It contains a URA3 marker for yeast selection.
References

Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568