Planned maintenance

We must perform activities that will impact the ability to place orders on our website. Starting Friday, May 21, at 5:00 p.m. Eastern to Sunday, May 23, at 5:00 p.m. Eastern, you will not be able to order products on our website. You will still be able to browse and access your account. We apologize for any inconvenience this work may cause.

pCarrier 3 (416) (ATCC® SB-1004)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

View Permits

Designations pCarrier 3 (416)
Depositors R Weiss, Massachusetts Institute of Technology
Applications A carrier vector in the ATCC® Synthetic Biology Yeast Tool Kit designed for use in the construction of a single transcription unit and gene circuit.
Vector Total size of construct (kb): 5.163
Vector type: Yeast low-copy vector
Markers: ampR
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Related Products ATCC SB-1005
ATCC SB-1024
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments The vector is a Saccharomyces cerevisiae/Escherichia coli shuttle vector. It contains a URA3 marker for yeast selection.

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436