3C9-D11-H11 (ATCC® CRL-1745)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Mice were immunized with purified PPV.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV)
Cellular Products
immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Mice were immunized with purified PPV.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody has both virus neutralization and hemagglutinating activities.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor RA Van Deusen
References

8th Proc. Intl. Pig. Vet. Soc. Gent, Belgium: 1984.

Katz JB, van Deusen RA. Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system. J. Virol. Methods 12: 193-198, 1985. PubMed: 3009510

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

8th Proc. Intl. Pig. Vet. Soc. Gent, Belgium: 1984.

Katz JB, van Deusen RA. Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system. J. Virol. Methods 12: 193-198, 1985. PubMed: 3009510