Chlamydophila pneumoniae (ATCC® VR-2282)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen

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Classification Chlamydiaceae, Chlamydophila
Deposited As Chlamydia pneumoniae Grayston et al.
Agent Chlamydophila pneumoniae
Strain TWAR strain TW-183
Respiratory research
Positive for Mycoplasma Yes
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Note that activities with high potential for aerosol production require BSL 3 facilities and practices.

For a time, prior to the acceptance of the C. pneumoniae strains as a separate species, the strains were refered to as TWAR, after the isolates TW-183 and AR-39. AR-39 is available as ATCC 53592

The inclusions are iodine stain negative (contain no glycogen).

Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour.

Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL cycloheximide. Incubate at 37°C for 72 hours.

Infection of TC is enhanced by subconfluent monolayer of cells.

This preparation has tested positive for Mycoplasma contamination.

The inclusions are iodine stain negative (contain no glycogen). The TWAR strains (now C. pneumoniae) of Chlamydia are named after TW183 and AR39.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies
Recommended Host
HEp-2 cells (ATCC® CCL-23™)
Growth Conditions

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90% - 100% confluent

Incubation: 3 days at 35°C, a 5% CO2 in air atmosphere is recommended

Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies
Name of Depositor G Galloway, T Grayston
U.S. Patent
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Special Collection NCRR Contract
Source Conjunctiva of a child, Taiwan, 1965
Year of Origin 1965

Grayston JT, et al. Chlamydia pneumoniae sp. nov. for Chlamydia sp. strain TWAR. Int. J. Syst. Bacteriol. 39: 88-90, 1989.

Kuo CC, Grayston JT. Chlamydia spp. strain TWAR: A newly recognized organism associated with atypical pneumonia and other respiratory infections. Clin. Microbiol. Newsletter 10: 137-140, 1988.

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462

type strain

Cross References

Nucleotide (GenBank) : AJ012174 Chlamydophila pneumoniae partial rnpB gene for RNase P RNA subunit.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation