Human herpesvirus 2 (ATCC® VR-1779)

Classification: Herpesviridae, Simplexvirus  /  Product Format: frozen 1 mL per vial

Classification Herpesviridae, Simplexvirus
Agent Human herpesvirus 2
Strain ATCC-2011-2
Common Name Herpes simplex 2
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions  -70°C or colder
Effect on Host
CPE, refractile cell rounding
CPE, refractile cell rounding
Recommended Host

Production Host:  Vero (ATCC® CCL-81)

Vero (ATCC CCL-81)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium, rinse monolayer with plain EMEM. Inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days in a humidified 5% CO2 atmosphere
Effect on Host
CPE, refractile cell rounding
CPE, refractile cell rounding
Name of Depositor ATCC
Source
Clinical sample from a patient positive for HSV-2 in Virginia, July 2011