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Human adenovirus 11

VR-12

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Human adenovirus 11 strain Slobitski was isolated in Massachusetts from human feces. This animal virus is propagated in KB cells (ATCC CCL-17) and has applications in respiratory research.
Product category
Viruses
Product type
Animal virus
Classification
Adenoviridae, Mastadenovirus, Human mastadenovirus B
Strain designation
Slobitski
Genome sequenced strain
Yes
Isolation source
Feces, human.
Geographical isolation
United States; Massachusetts
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Respiratory research

Characteristics

Comments
Agglutinates rhesus RBC. Weakly oncogenic. Contains soluble group-specific CF antigen. Efforts at ATCC to detect polio by antibody-antigen reaction in VR-12 have yielded negative results.
Mycoplasma contamination
Not detected

Handling information

Effects
cell rounding; cell sloughing; CPE
Complete medium
EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Handling notes
Agglutinates rhesus RBC. Weakly oncogenic. Contains soluble group-specific CF antigen. Efforts at ATCC to detect polio by antibody-antigen reaction in ATCC VR-12 have yielded negative results.
Key abbreviations
CF, Complement fixation
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
KB, Human oral epidermal carcinoma cells
PBS, Phosphate-buffered saline
RBC, Red blood cell

Quality control specifications

Volume
1.0 mL
Verification method
Whole-genome Sequencing

History

Deposited as
Adenovirus type 11
Depositors
M Schaeffer, DA Stevens
Type of isolate
Human
Cross references
GenBank U52569 Human adenovirus type 11 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Rowe WP, Hartley JW, Huebner RJ. Additional serotypes of the APC virus group. Proc. Soc. Exp. Biol. Med. 91: 260-262, 1956. PubMed: 13297771

Kibrick S, Melendez L, Enders JF. Clinical associations of enteric viruses with particular reference to agents exhibiting properties of the ECHO group. Ann. NY Acad. Sci. 67: 311-325, 1957. PubMed: 13411969

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

View All Curated Citations for this Product

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