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MUG-CC1 (ATCC® CRL-3327)

Organism: Homo sapiens, human  /  Tissue: Primary site: Clivus  /  Disease: Chordoma tumor

Permits and Restrictions

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Organism Homo sapiens, human
Tissue Primary site: Clivus
Product Format frozen 1.0 mL
Morphology mesenchymal-like, physaliferous
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Chordoma tumor
Age 72 years
Gender male
Ethnicity Caucasian
Applications
Use as a model for chordoma which is a rare slow-growing tumor

The Chordoma Foundation may be able to offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information.
Storage Conditions liquid nitrogen vapor phase
Images
Antigen Expression HLA: A 24:02
Comments Nuclear expression of brachyury and CD24 (verified with immunofluorescence and PCR). This cell line was accessioned with the support of the Chordoma Foundation, a nonprofit organization working to improve the lives of chordoma patients by accelerating research to develop effective treatments for chordoma.
Complete Growth Medium

The base medium for this cell line is Iscove's Modified Dulbecco's Medium (IMDM; ATCC® No. 30-2005): RPMI-1640 Medium (ATCC® No. 30-2001) at a 4 to 1 ratio. To 500 mL IMDM:RPMI 1640 (4:1) add the following components to make the complete medium:

  • 56 mL  FBS (ATCC® No. 30-2020), final concentration of 10%
  • 1% L-glutamine (ATCC® No. 30-2214), final concentation of 1%
  • 5.6 mL L-Glutamine (Gibco cat# 25030-081)
  • 5.6 mL ITS-G (Gibco cat# 41400-045)
Subculturing
It is recommended to subculture the cells at maximum 85% confluency. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    Cultures can be established between 2 x 104 and 6 x 104 viable cells/cm2.
  6. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 2 X 104 and 2.0 X 105 cell/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial approximately 1 to 2 x 106 cells
Volume 1.0 mL
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 9,14
D5S818: 11,12
D7S820: 8,11
TH01: 9.3
TPOX: 8,11
vWA: 16,18
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viability ≥ 50%
Population Doubling Time about 12 days
Name of Depositor B Rinner
Year of Origin 2014
References

Geller V, et al. Establishment of clival chordoma cell line MUG-CC1 and lymphoblastoid cells as a model for potential new treatment strategies. Sci Rep 6: 24195. doi: 10.1038/srep24195, 2016. PubMed: 27072875

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Geller V, et al. Establishment of clival chordoma cell line MUG-CC1 and lymphoblastoid cells as a model for potential new treatment strategies. Sci Rep 6: 24195. doi: 10.1038/srep24195, 2016. PubMed: 27072875