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H36.12b [Pixie 12b] (ATCC® CRL-2446)

Organism: Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage)  /  Cell Type: Macrophage  / 

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Organism Mus musculus (macrophage tumor cell line); Mus musculus (peritoneal macrophage), mouse(macrophage tumor cell line); mouse (peritoneal macrophage)
Cell Type Macrophage
Product Format frozen
Morphology macrophage
Culture Properties mixed: adherent and suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
Unlike H36.12j (ATCC CRL-2449), the cells do not produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation.
Storage Conditions liquid nitrogen vapor phase
Derivation
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
Comments
This cell line was established by P.A. Campbell and E.P. Canono in 1991. Percoll gradient purified, proteose peptone elicited peritoneal macrophage cells from C57BL/6N mice were fused with drug selected P388D1 mouse macrophage tumor cells.
Unlike H36.12j (ATCC CRL-2449), the cells do not produce tumor necrosis factor alpha (TNF alpha) upon direct beryllium stimulation.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated iron supplemented bovine calf serum to a final concentration of 10%
  • Subculturing Add fresh medium every 2 to 3 days (depending on cell density). Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 x 105 viable cells/mL.

    Subcultivation Ratio: 1:5 every 3 to 4 days is recommended
    Medium Renewal: Every 2 to 3 days
    Cryopreservation Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

    Culture Conditions
    Temperature: 37°C
    Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
    Name of Depositor RT Sawyer
    Deposited As mouse
    Year of Origin 1991
    References

    Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Canono EP, Campbell PA. Production of mouse inflammatory hybridomas. J. Tissue Culture Methods 14: 3-8, 1992.

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.