WI-26 VA4 (ATCC® CCL-95.1)

Organism: Homo sapiens, human  /  Cell Type: SV40 transformed  /  Tissue: lung  / 

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Organism Homo sapiens, human
Tissue lung
Cell Type SV40 transformed
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 3 month gestation
Gender male
Ethnicity Caucasian
Applications
The cells are positive for SV40 neo (T) antigen and SV40 transplantation antigen.
Storage Conditions liquid nitrogen vapor temperature
Karyotype Chromosome Frequency Distribution 49 Cells: 2n = 46
Karyotype unstable within triploid stemline range of 67 to 71. Ninety-six percent of the cells have a large chromosome with a submedian centromere. Approximately 52% of the cells have one or more secondary constrictions; 42% of the cells examined had breaks.
Derivation
WI-26 VA4 is an SV40 virus transformed derivative of WI-26 a human diploid fibroblast cell line from embryonic lung tissue. After initial infection and crisis-phase of SV40-transformation, the cells underwent a change in morphology. The cells are positive for SV40 neo (T) antigen and SV40 transplantation antigen.
Clinical Data
male
Caucasian
3 month gestation
Comments
WI-26 VA4 is an SV40 virus transformed derivative of WI-26 a human diploid fibroblast cell line from embryonic lung tissue. After initial infection and crisis-phase of SV40-transformation, the cells underwent a change in morphology. The cells are positive for SV40 neo (T) antigen and SV40 transplantation antigen.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:2 to 1:5
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 5% Carbon dioxide (CO2)
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 9,11
D5S818: 11,12
D7S820: 10,13
THO1: 6,7
TPOX: 10,11
vWA: 17,20
Isoenzymes
G6PD, B
Name of Depositor AJ Girardi
Deposited As Homo sapiens
References

Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed: 13905659

Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed: 14315085

Girardi AJ, et al. SV40-induced transformation of human diploid cells: crisis and recovery. J. Cell. Comp. Physiol. 65: 69-84, 1965.

Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed: 13905659

Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed: 14315085

Girardi AJ, et al. SV40-induced transformation of human diploid cells: crisis and recovery. J. Cell. Comp. Physiol. 65: 69-84, 1965.

Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.