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Thermoanaerobacterium thermosulfurigenes (Schink and Zeikus) Lee et al. (ATCC® 33743™)

Strain Designations: 4B [DSM 2229]  /  Type Strain: yes  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Clostridium thermosulfurigenes Schink and Zeikus
Strain Designations 4B [DSM 2229]
Application
Hydrolyzes starch
Produces beta-amylase
Produces ethyl alcohol ethanol
Produces exo-poly-alpha-galacturonosidase polygalacturonate hydrolase
Produces pectinesterase pectin methylesterase
Produces thermostable beta-amylase
Produces ethanol from starch
Converts thiosulfate to elemental sulfur
Biotechnology
Isolation
Microbial mat
United States
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format freeze-dried
Preceptrol® no
Type Strain yes RefValidation list no. 12. Int. J. Syst. Bacteriol. 33: 896-897, 1983. , RefLee YE, et al. Taxonomic distinction of saccharolytic thermophilic anaerobes: description of Thermoanaerobacterium xylanolyticum gen. nov., sp. nov., and Thermoanaerobacterium saccharolyticum gen. nov., sp. nov.; reclassification of Thermoanaerobium brockii, Clostridium thermosulfurogenes, and Clostridium thermohydrosulfuricum E100-69 as Thermoanaerobacter brockii comb. nov., Thermoanaerobacterium thermosulfurigenes comb. nov., and Thermoanaerobacter thermohydrosulfuricus comb. nov., respectively; and transfer of Clostr. Int. J. Syst. Bacteriol. 43: 41-51, 1993. , (type strain)
Medium ATCC® Medium 1398: Modified low phosphate buffered basal medium
Growth Conditions
Temperature: 60.0°C
Atmosphere: Anaerobic
Name of Depositor JG Zeikus
Special Collection National Park Service Special Collection (NPS)
Isolation
Microbial mat
United States
Geographical Isolation Yellowstone National Park
References

Hyun HH, Zeikus JG. General biochemical characterization of thermostable extracellular beta-amylase from Clostridium thermosulfurogenes. Appl. Environ. Microbiol. 49: 1162-1167, 1985.

Zeikus JG, Hyun HH. Co-culture production of thermostable enzymes and ethanol. US Patent 4,604,352 dated Aug 5 1986

Zeikus JG. Thermophile isolate having thermostable hydrolytic activity. US Patent 4,572,898 dated Feb 25 1986

Zeikus JG. Novel thermophile isolate having thermostable hydrolytic activity. US Patent 4,721,676 dated Jan 26 1988

Zeikus JG, Hyun HH. Thermostable beta-amylase. US Patent 4,647,538 dated Mar 3 1987

Zeikus JG, Hyun HH. Regulation and enhancement of enzyme production. US Patent 4,737,459 dated Apr 12 1988

Validation list no. 12. Int. J. Syst. Bacteriol. 33: 896-897, 1983.

Lee YE, et al. Taxonomic distinction of saccharolytic thermophilic anaerobes: description of Thermoanaerobacterium xylanolyticum gen. nov., sp. nov., and Thermoanaerobacterium saccharolyticum gen. nov., sp. nov.; reclassification of Thermoanaerobium brockii, Clostridium thermosulfurogenes, and Clostridium thermohydrosulfuricum E100-69 as Thermoanaerobacter brockii comb. nov., Thermoanaerobacterium thermosulfurigenes comb. nov., and Thermoanaerobacter thermohydrosulfuricus comb. nov., respectively; and transfer of Clostr. Int. J. Syst. Bacteriol. 43: 41-51, 1993.

type strain

algal mat, Yellowstone National Park

Validation list no. 12. Int. J. Syst. Bacteriol. 33: 896-897, 1983.

Lee YE, et al. Taxonomic distinction of saccharolytic thermophilic anaerobes: description of Thermoanaerobacterium xylanolyticum gen. nov., sp. nov., and Thermoanaerobacterium saccharolyticum gen. nov., sp. nov.; reclassification of Thermoanaerobium brockii, Clostridium thermosulfurogenes, and Clostridium thermohydrosulfuricum E100-69 as Thermoanaerobacter brockii comb. nov., Thermoanaerobacterium thermosulfurigenes comb. nov., and Thermoanaerobacter thermohydrosulfuricus comb. nov., respectively; and transfer of Clostr. Int. J. Syst. Bacteriol. 43: 41-51, 1993.

type strain

Cross References

Nucleotide (GenBank) : M22471 C. thermosulfurogenes thermophilic beta-amylase gene, complete cds.

Nucleotide (GenBank) : L09171 Thermoanaerobacterium thermosulfurigenes (ATCC 33743) 16S ribosomal RNA (16S rRNA) gene.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • This material is distributed for scientific and educational research purposes only and is part of the National Park Service Special Collection. A signed National Park Service MTA is both required prior to shipment to customers proposing a non-commercial use. A separate agreement is required from the National Park Service for customers proposing a commercial use.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS