Eggerthella lenta (Eggerth) Wade et al.

1. Open vial according to enclosed instructions. 
2. Under anaerobic conditions, withdraw 0.5 mL of #1490 broth from a single test tube (5 to 6 mL), with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a pre-reduced Brucella blood plate and a #260 slant. An aerobic blood plate may also be streaked to check for purity.
5. Incubate the tubes and plate under anaerobic conditions at 37°C for 24 to 48 hours. Incubate the purity plate aerobically at 37°C. Turbid growth is evident in the broth at 24 to 48 hours.

ANAEROBIC CONDITIONS:   

Anaerobic conditions for transfer may be obtained by either of the following:

• Use of an anaerobic gas chamber.
• Placement of test tubes under a gassing cannula system hooked to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

• Loose screw caps on test tubes in anaerobic chamber.
• Loose screw caps on test tubes in an activated anaerobic gas pack jar.
• Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.

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