Primary Airway Cells in 3-D Models: Optimizing Culture at the Air-liquid Interface Webinar

Advanced in vitro airway models promise to provide relevant applications for use in human respiratory research; however, the numerous variables associated in generating these advanced models can cause incomplete or inconsistent differentiation. To reduce these variables, ATCC scientists set out to identify optimal methods for constructing airway models consisting of fully differentiated human bronchial tracheal epithelial cells (HBECs) cultured under air-liquid interface (ALI) conditions. Within this ALI model, we evaluated different production lots of primary HBECs from ATCC and other commercial vendors, hTERT immortalized HBECs, and various commercial medias. In this webinar, we will elucidate techniques and procedures that can help you reliably generate 3-D airway models with consistent full epithelial differentiation for your drug development studies, toxicological testing, and basic research. After the presentation, join us for a lively discussion as our R&D scientists answer your questions.

Key Points

• Adoption of 3-D models for toxicological studies, drug discovery, and basic research is growing.
• Numerous variables can confound experimental results and thereby slow the pace of your research.
• This study demonstrates optimized methods to generate airway models with appropriate model morphology and epithelial differentiation and help to reduce inter-experimental variability.