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Chlamydia trachomatis

VR-902B

Product category
Bacteria
Classification
Chlamydiaceae, Chlamydia
Strain designation
Lymphogranuloma venereum (LGV II) strain 434
Type strain
No
Isolation source
Bubo from human with LGV.
Applications
Infectious disease research
Sexually transmitted disease research
Product format
Frozen
Storage conditions
-70°C or colder
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Biosafety Icon BSL 3

Biosafety level 3 is applicable to any facility where work is performed with indigenous or exotic agents that may cause serious or potentially lethal disease through inhalation. Laboratory personnel must receive specific training in handling pathogenic, potentially lethal agents and must be supervised by scientists competent in handling infectious agents. ATCC determines the biosafety level of a material based on our risk assessment and as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Sexually Transmitted Disease Research

Characteristics

Comments
FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
LGV II strain 434 was initially accessioned as an egg passaged preparation (VR-902) and subsequently adapted to cell culture at ATCC and reaccessioned as VR-902B. Dilute high titer stocks to avoid cell toxicity.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Mycoplasma contamination
Not detected

Handling information

Host
This product information is not available online. Due to the nature of this product, we only provide this information to customers who have purchased this biosafety level 3 product. If you have purchased this product, please contact Technical Services for this product information.
Effects
This product information is not available online. Due to the nature of this product, we only provide this information to customers who have purchased this biosafety level 3 product. If you have purchased this product, please contact Technical Services for this product information.
Complete medium
This product information is not available online. Due to the nature of this product, we only provide this information to customers who have purchased this biosafety level 3 product. If you have purchased this product, please contact Technical Services for this product information.
Temperature
This product information is not available online. Due to the nature of this product, we only provide this information to customers who have purchased this biosafety level 3 product. If you have purchased this product, please contact Technical Services for this product information.
Atmosphere
This product information is not available online. Due to the nature of this product, we only provide this information to customers who have purchased this biosafety level 3 product. If you have purchased this product, please contact Technical Services for this product information.
Incubation
This product information is not available online. Due to the nature of this product, we only provide this information to customers who have purchased this biosafety level 3 product. If you have purchased this product, please contact Technical Services for this product information.
Recommendation for infection
This product information is not available online. Due to the nature of this product, we only provide this information to customers who have purchased this biosafety level 3 product. If you have purchased this product, please contact Technical Services for this product information.
Handling notes
This product information is not available online. Due to the nature of this product, we only provide this information to customers who have purchased this biosafety level 3 product. If you have purchased this product, please contact Technical Services for this product information.
Key abbreviations
This product information is not available online. Due to the nature of this product, we only provide this information to customers who have purchased this biosafety level 3 product. If you have purchased this product, please contact Technical Services for this product information.

Quality control specifications

Volume
1.0 mL

History

Deposited as
Chlamydia trachomatis (Busacca) Rake
Depositors
J Schachter, adapted at ATCC
Type of isolate
Human
Cross references
GenBank U68443 Chlamydia trachomatis strain L2/434/BU 16S and 23S ribosomal RNA operon, complete sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618

Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529

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